Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties
文献类型：期刊论文
| 作者 | Han, Subing2,3; Zhao, Jialin1; Liu, Ping3; Wang, Kang2; Qin, Song2 ; Zhao, Zhenjun3; Cui, Yulin2
|
| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2022-08-01 |
| 卷号 | 20期号:8页码:11 |
| 关键词 | Porphyridium purpureum chloroplast transformation system microparticle bombardment antimicrobial peptides NZ2114 Piscidin-4 inhibitory effect |
| DOI | 10.3390/md20080484 |
| 通讯作者 | Cui, Yulin(yulincui@yic.ac.cn) |
| 英文摘要 | To solve the problem of antibiotic abuse in aquaculture and to utilize the application potential of antimicrobial peptides (AMPs), a chloroplast transformation system of Porphyridium purpureum was successfully constructed for effectively expressing two exogenous AMPs. The endogenous fragments of 16S rDNA/trnA-23S rDNA were used as flanking fragments for the homologous recombination in the chloroplast genome. Two AMPs encoded by the transformation vector were controlled by the native promoter psbB in a polycistron. The plasmids were transferred into P. purpureum via particle bombardment and the transformation vectors were screened using phosphinothricin (bar), a dominant selection marker under the control of the psbA promoter. Subsequently, in the positive transformed colonies, the exogenous fragments were found to be inserted in the flanking fragments directionally as expected and two foreign AMPs were successfully obtained. Finally, two exogenous peptides with antibacterial properties were obtained from the transformed strain. The two AMPs expressed by the transformed strain were shown to have similar inhibitory effects to antibiotics by inhibition tests. This suggested that AMPs can be introduced into aquaculture using baited microalgae, providing new ideas and ways to solve a series of aquaculture diseases caused by bacteria. |
| WOS关键词 | TETRASELMIS SUBCORDIFORMIS PRASINOPHYCEAE ; PLECTASIN ; NZ2114 ; TRANSFORMATION ; CHLOROPHYTA ; VACCINES ; PROTEIN |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000846719300001 |
| 资助机构 | National Natural Science Foundation of China ; Project of Innovation and Development of Marine Economy |
| 源URL | [http://ir.yic.ac.cn/handle/133337/31664]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Cui, Yulin |
| 作者单位 | 1.Yantai Marine Econ Res Inst, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China 3.Yantai Univ, Coll Life Sci, Yantai 264000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Subing,Zhao, Jialin,Liu, Ping,et al. Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties[J]. MARINE DRUGS,2022,20(8):11. |
| APA | Han, Subing.,Zhao, Jialin.,Liu, Ping.,Wang, Kang.,Qin, Song.,...&Cui, Yulin.(2022).Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties.MARINE DRUGS,20(8),11. |
| MLA | Han, Subing,et al."Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties".MARINE DRUGS 20.8(2022):11. |
入库方式： OAI收割
来源：烟台海岸带研究所
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