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In situ methods of plant-microbial interactions for nitrogen in rhizosphere

文献类型：期刊论文

作者Liu, Min1,2; Adl, Sina3; Cui, Xiaoyong4,5; Tian, Yuqiang6; Xu, Xingliang1,4; Kuzyakov, Yakov1,7
刊名RHIZOSPHERE
出版日期2020-03-01
卷号13页码:6
关键词In situ labeling Microbial ecology Nitrogen uptake Plant-microbial competition Rhizosphere
DOI10.1016/j.rhisph.2020.100186
通讯作者Xu, Xingliang(xuxingl@hotmail.com)
英文摘要Nitrogen (N) is an essential element for plants and microorganisms. In most terrestrial ecosystems, N is a major nutrient limiting plant growth, leading to strong competition for N between roots and rhizosphere microorganisms. Given the importance of N, investigations of plant-microbial competition for N over time and space are a prerequisite for better understanding of net primary production, nutrient cycling and root-microbial interactions. Here we proposed an in situ approach using stable isotopes (N-15 and C-13) to quantify plant-microbial N and C flows. The approach is based on N-15 and C-13 partition between plants and microorganisms and allows to investigate the chemical (N forms), spatial (labeling depths) and temporal (seasons) parameters for better understanding ecological functions of belowground systems. In this method, quadrats are selected and labeled with N-15 tracers (organic N forms also labeled with C-13), i.e., introducing N-15 labeled NH4+ or NO3-, or N-15 and C-13 dual labeled amino acids allows to quantifying uptake preferences by plants and/or microorganisms. Detailed calculations of the amount of N-15 and C-13 label are presented. Such approach is necessary for comparisons between studies, and to advance our knowledge of rhizosphere interactions in terrestrial ecosystems.
WOS关键词ORGANIC-NITROGEN ; STABLE-ISOTOPES ; INORGANIC NITROGEN ; EXTRACTION METHOD ; SOIL MICROBES ; AMINO-ACIDS ; GROWTH ; MICROORGANISMS ; BIOMASS ; CARBON
资助项目CAS Strategic Priority Research Programme[XDA20050104] ; National Key Research and Development Program of China[2016YFC0501802] ; National Key Research and Development Program of China[2017YFA0604802] ; National Natural Science Foundation of China[41877089]
WOS研究方向Agriculture ; Plant Sciences
语种英语
WOS记录号WOS:000521128100010
出版者ELSEVIER
资助机构CAS Strategic Priority Research Programme ; National Key Research and Development Program of China ; National Natural Science Foundation of China
源URL[http://ir.igsnrr.ac.cn/handle/311030/133426]  
专题中国科学院地理科学与资源研究所
通讯作者Xu, Xingliang
作者单位1.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, 11A Datun Rd, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 101408, Peoples R China
3.Univ Saskatchewan, Coll Agr & Bioresources, Dept Soil Sci, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada
4.Chinese Acad Sci, CAS Ctr Excellence Tibetan Plateau Earth Sci, Beijing 100101, Peoples R China
5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 101408, Peoples R China
6.Beijing Normal Univ, Fac Geog Sci, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China
7.Univ Gottingen, Dept Soil Sci Temperate Ecosyst, Busgenweg 2, D-37077 Gottingen, Germany
推荐引用方式
GB/T 7714		 Liu, Min,Adl, Sina,Cui, Xiaoyong,et al. In situ methods of plant-microbial interactions for nitrogen in rhizosphere[J]. RHIZOSPHERE,2020,13:6.
APA Liu, Min,Adl, Sina,Cui, Xiaoyong,Tian, Yuqiang,Xu, Xingliang,&Kuzyakov, Yakov.(2020).In situ methods of plant-microbial interactions for nitrogen in rhizosphere.RHIZOSPHERE,13,6.
MLA Liu, Min,et al."In situ methods of plant-microbial interactions for nitrogen in rhizosphere".RHIZOSPHERE 13(2020):6.

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来源：地理科学与资源研究所

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