Cell Nucleus Targeting for Living Cell Extraction of Nucleic Acid Associated Proteins with Intracellular Nanoprobes of Magnetic Carbon Nanotubes
文献类型：期刊论文
| 作者 | Zhang, Yi1,2; Hu, Zhengyan1,2; Qin, Hongqiang1,2; Liu, Fangjie1,2; Cheng, Kai1,2; Wu, Ren'an1; Zou, Hanfa1 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2013-08-06 |
| 卷号 | 85期号:15页码:7038-7043 |
| ISSN号 | 0003-2700 |
| 产权排序 | 1,1 |
| 通讯作者 | 邹汉法 ; 吴仁安 |
| 英文摘要 | since nanoparticles could be ingested by cells naturally and target at a specific cellular location as designed, the extraction of intracellular proteins from living cells for large-scale analysis by nanoprobes seems to be ideally possible. nucleic acid associated proteins (naap) take the crucial position during biological processes in maintaining and regulating gene structure and gene related behaviors, yet there are still challenges during the global investigation of intracellular naap, especially from living cells. in this work, a strategy to extract intracellular proteins from living cells with the magnetic carbon nanotube (omwcnt@fe3o4) as an intracellular probe is developed, to achieve the high throughput analysis of naap from living human hepatoma bel-7402 cells with a mass spectrometry-based proteomic approach. due to the specific intracellular localization of the magnetic carbon nanotubes around nuclei and its strong interaction with nucleic acids, the highly efficient extraction was realized for cellular naap from living cells, with the capability of identifying 2383 intracellular naap from only ca. 10 000 living cells. this method exhibited potential applications in dynamic and in situ analysis of intracellular proteins. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | in-vitro ; mass-spectrometry ; gene-expression ; dna ; delivery ; proteomics ; binding |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000323014000012 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117543]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yi,Hu, Zhengyan,Qin, Hongqiang,et al. Cell Nucleus Targeting for Living Cell Extraction of Nucleic Acid Associated Proteins with Intracellular Nanoprobes of Magnetic Carbon Nanotubes[J]. analytical chemistry,2013,85(15):7038-7043. |
| APA | Zhang, Yi.,Hu, Zhengyan.,Qin, Hongqiang.,Liu, Fangjie.,Cheng, Kai.,...&Zou, Hanfa.(2013).Cell Nucleus Targeting for Living Cell Extraction of Nucleic Acid Associated Proteins with Intracellular Nanoprobes of Magnetic Carbon Nanotubes.analytical chemistry,85(15),7038-7043. |
| MLA | Zhang, Yi,et al."Cell Nucleus Targeting for Living Cell Extraction of Nucleic Acid Associated Proteins with Intracellular Nanoprobes of Magnetic Carbon Nanotubes".analytical chemistry 85.15(2013):7038-7043. |
入库方式： OAI收割
来源：大连化学物理研究所
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