Mesenchymal Stem Cell and Chondrocyte Fates in a Multishear Microdevice Are Regulated by Yes-Associated Protein
文献类型：期刊论文
| 作者 | Zhong, Weiliang1; Tian, Kang1; Zheng, Xifu1; Li, Linan1; Zhang, Weiguo1; Wang, Shouyu1; Qin, Jianhua2 |
| 刊名 | stem cells and development
 |
| 出版日期 | 2013-07-01 |
| 卷号 | 22期号:14页码:2083-2093 |
| ISSN号 | 1547-3287 |
| 产权排序 | 2,7 |
| 通讯作者 | weiguozhang |
| 英文摘要 | mechanical cues exert considerable influence on the fates of stem cells and terminally differentiated chondrocytes. the elucidation of the interactions between cell fate and mechanical cues in nuclear mechanotransduction will provide new clues to modulate tissue homeostasis and regeneration. in this study, we used an integrated microfluidic perfusion device to simultaneously generate multiple-parameter fluid shear stresses to investigate the role of fluid flow stimuli in the regulation of yes-associated protein (yap) expression and the fates of mesenchymal stem cells (mscs) and primary chondrocytes. yap expression was regulated by the level of fluid flow stimulus in both mscs and chondrocytes. an increase in the magnitude of stimulation enhanced the expression of yap, ultimately resulting in an increase in osteogenesis and a decrease in adipogenesis for mscs, and initiating dedifferentiation for chondrocytes. cytochalasin d not only repressed nuclear yap accumulation in the flow state, but also abrogated flow-induced effects on msc differentiation and the chondrocyte phenotype, resulting in msc adipogenesis and the maintenance of the chondrocyte phenotype. our findings reveal the connection between yap and msc/chondrocyte fates in a fluid flow-induced mechanical microenvironment and provide new insights into the mechanisms by which mechanical cues regulate the fates of mscs and chondrocytes. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | cell & tissue engineering ; hematology ; medicine, research & experimental ; transplantation |
| 研究领域[WOS] | cell biology ; hematology ; research & experimental medicine ; transplantation |
| 关键词[WOS] | organ size control ; shear-stress ; hippo pathway ; osteogenic differentiation ; tissue regeneration ; interstitial flow ; focal adhesion ; growth-control ; self-renewal ; proliferation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000321186700010 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117545]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Med Univ, Affiliated Hosp 1, Dept Orthopaed, Dalian 116011, Peoples R China 2.Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, Weiliang,Tian, Kang,Zheng, Xifu,et al. Mesenchymal Stem Cell and Chondrocyte Fates in a Multishear Microdevice Are Regulated by Yes-Associated Protein[J]. stem cells and development,2013,22(14):2083-2093. |
| APA | Zhong, Weiliang.,Tian, Kang.,Zheng, Xifu.,Li, Linan.,Zhang, Weiguo.,...&Qin, Jianhua.(2013).Mesenchymal Stem Cell and Chondrocyte Fates in a Multishear Microdevice Are Regulated by Yes-Associated Protein.stem cells and development,22(14),2083-2093. |
| MLA | Zhong, Weiliang,et al."Mesenchymal Stem Cell and Chondrocyte Fates in a Multishear Microdevice Are Regulated by Yes-Associated Protein".stem cells and development 22.14(2013):2083-2093. |
入库方式： OAI收割
来源：大连化学物理研究所
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