Polyacrylamide Gel with Switchable Trypsin Activity for Analysis of Proteins
文献类型：期刊论文
| 作者 | Liu, Fangjie1,2; Ye, Mingliang1; Wang, Chunli1,2; Hu, Zhengyan1,2; Zhang, Yi1,2; Qin, Hongqiang1,2; Cheng, Kai1,2; Zou, Hanfa1 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2013-08-06 |
| 卷号 | 85期号:15页码:7024-7028 |
| ISSN号 | 0003-2700 |
| 产权排序 | 1,1 |
| 通讯作者 | 邹汉法 ; 叶明亮 |
| 英文摘要 | trypsin was immobilized on a variety of materials to improve digestion efficiency. however, because the immobilized trypsin will digest proteins during electrophoresis, direct immobilization of active trypsin in polyacrylamide gel will compromise the protein separation. to overcome this problem, here we report a novel polyacrylamide gel with switchable trypsin activity. it was prepared by copolymerization of the peg-trypsin-aprotinin complex during the gel-casting step. because the inhibitor aprotinin binds strongly with trypsin at alkaline ph, this novel gel does not display hydrolytic activity during electrophoresis. after electrophoresis, the activity of trypsin embedded in gel could be recovered by simply washing away the bound inhibitor at a low ph. it was demonstrated that this unique switchable activity design allowed high resolution of the complex protein mixture during electrophoresis and highly efficient digestion of the separated proteins in situ in the gel after electrophoresis. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | mass-spectrometry ; identification ; proteomics ; digestion ; membranes |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000323014000009 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117567]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Fangjie,Ye, Mingliang,Wang, Chunli,et al. Polyacrylamide Gel with Switchable Trypsin Activity for Analysis of Proteins[J]. analytical chemistry,2013,85(15):7024-7028. |
| APA | Liu, Fangjie.,Ye, Mingliang.,Wang, Chunli.,Hu, Zhengyan.,Zhang, Yi.,...&Zou, Hanfa.(2013).Polyacrylamide Gel with Switchable Trypsin Activity for Analysis of Proteins.analytical chemistry,85(15),7024-7028. |
| MLA | Liu, Fangjie,et al."Polyacrylamide Gel with Switchable Trypsin Activity for Analysis of Proteins".analytical chemistry 85.15(2013):7024-7028. |
入库方式： OAI收割
来源：大连化学物理研究所
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