A six-plex proteome quantification strategy reveals the dynamics of protein turnover
文献类型：期刊论文
| 作者 | Wang, Fangjun; Cheng, Kai; Wei, Xiaoluan; Qin, Hongqiang; Chen, Rui; Liu, Jing; Zou, Hanfa |
| 刊名 | scientific reports
 |
| 出版日期 | 2013-05-10 |
| 卷号 | 3页码:18271 |
| ISSN号 | 2045-2322 |
| 产权排序 | 1,1 |
| 通讯作者 | 王方军 ; 邹汉法 |
| 英文摘要 | ms1 full scan based quantification is one of the most popular approaches for large-scale proteome quantification. typically only three different samples can be differentially labeled and quantified in a single experiment. here we present a two stages stable isotope labeling strategy which allows six different protein samples (six-plex) to be reliably labeled and simultaneously quantified at ms1 level. briefly in the first stage, isotope lysine-d0 (k0) and lysine-d4 (k4) are in vivo incorporated into different protein samples during cell culture. then in the second stage, three of k0 and k4 labeled protein samples are digested by lysine c and in vitro labeled with light (2ch(3)), medium (2cd(2)h), and heavy (2(13)cd(3)) dimethyl groups, respectively. we demonstrated that this six-plex isotope labeling strategy could successfully investigate the dynamics of protein turnover in a high throughput manner. |
| WOS标题词 | science & technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | multidisciplinary sciences |
| 研究领域[WOS] | science & technology - other topics |
| 关键词[WOS] | multiplexed quantitative proteomics ; mass-spectrometry ; peptide identification ; accurate ; cells ; expression |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318652900004 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117587]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fangjun,Cheng, Kai,Wei, Xiaoluan,et al. A six-plex proteome quantification strategy reveals the dynamics of protein turnover[J]. scientific reports,2013,3:18271. |
| APA | Wang, Fangjun.,Cheng, Kai.,Wei, Xiaoluan.,Qin, Hongqiang.,Chen, Rui.,...&Zou, Hanfa.(2013).A six-plex proteome quantification strategy reveals the dynamics of protein turnover.scientific reports,3,18271. |
| MLA | Wang, Fangjun,et al."A six-plex proteome quantification strategy reveals the dynamics of protein turnover".scientific reports 3(2013):18271. |
入库方式： OAI收割
来源：大连化学物理研究所
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