Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells
文献类型：期刊论文
| 作者 | Liu, Yang1; Liu, Chang1,2; Nan, Feng3; Xiao, Jing4; Lian, Jian-chun5; He, Xin5; Guo, Xin1; Sun, Guang-wei1; Ma, Xiao-jun1 |
| 刊名 | journal of hard tissue biology
 |
| 出版日期 | 2013-04-01 |
| 卷号 | 22期号:2页码:241-247 |
| 关键词 | Cell co-culture Mesenchymal stem cell Microcapsule Neural differentiation |
| 产权排序 | 1,1 |
| 通讯作者 | 孙广炜 ; 马小军 |
| 英文摘要 | cell co-culture plays a key role in inducing the committed differentiation of stem cells in vitro, which mimics the specific tissue microenvironment in vivo by means of co-cultured inducing cells. however, the present cell co-culture systems limit the application of differentiated cells due to the safety and the scale of these systems. this study established a novel microcapsule co-culture system and investigated the probable effect of microencapsulated co-cultured neural cells on neural differentiation of mesenchymal stem cells (mscs) ex vivo. mouse bone marrow-derived mscs were co-cultured with shsy5y cells microencapsulated in alginate-polylysine-alginate (apa) microcapsules. the quantitative and qualitative analysis showed that the expression of nestin (nes) was significantly increased at both gene and protein levels in the microcapsule co-culture system compared with other systems without cell co-culture, which indicated that more neural progenitors were obtained from this novel microcapsule co-culture system. according to our results and the unique characteristics of microcapsules, it is possible that large-scale cell co-culture system based on the combination of microcapsule and bioreactor technologies will be developed to generate numerous mscs-derived neural progenitors for clinical applications in the future. |
| WOS标题词 | science & technology ; technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | engineering, biomedical |
| 研究领域[WOS] | engineering |
| 关键词[WOS] | transplantation ; expression ; therapy ; culture ; stroke ; growth ; injury ; rat |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318190200013 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117649]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Dalian Med Univ, Hosp 2, Dept Orthoped, Dalian, Peoples R China 4.Dalian Med Univ, Collage Stomatol, Dept Oral Basic Sci, Dalian, Peoples R China 5.Dalian Med Univ, Lab Med Coll, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yang,Liu, Chang,Nan, Feng,et al. Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells[J]. journal of hard tissue biology,2013,22(2):241-247. |
| APA | Liu, Yang.,Liu, Chang.,Nan, Feng.,Xiao, Jing.,Lian, Jian-chun.,...&Ma, Xiao-jun.(2013).Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells.journal of hard tissue biology,22(2),241-247. |
| MLA | Liu, Yang,et al."Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells".journal of hard tissue biology 22.2(2013):241-247. |
入库方式： OAI收割
来源：大连化学物理研究所
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