Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation
文献类型：期刊论文
| 作者 | Ma, Ying1,2; Zhang, Ying1; Wang, Yu1,2; Wang, Qiuyan3; Tan, Mingqian1; Liu, Yang1; Chen, Li1,2; Li, Na1,2; Yu, Weiting1; Ma, Xiaojun1 |
| 刊名 | journal of biomedical materials research part a
 |
| 出版日期 | 2013-04-01 |
| 卷号 | 101期号:4页码:1007-1015 |
| 关键词 | microencapsulation membrane thickness strength permeability cell proliferation |
| 产权排序 | 1,1 |
| 通讯作者 | 马小军 |
| 英文摘要 | cell microencapsulation is one of the promising strategies for in vitro production of proteins or in vivo delivery of therapeutic products. membrane thickness controls microcapsule strength and permeability, which may in return affect cell growth and metabolism. in this study, the strength, permeability, and encapsulated chinese hamster ovary cell proliferation and metabolism of four groups of microcapsules with different membrane thicknesses were investigated. it was found that increasing membrane thickness increases microcapsule strength, whereas decreases membrane permeability. during the first 6 days, cells within microcapsules with 10 m thickness membrane proliferated fast and could reach a cell density of 1.9 x 107 cells/ml microcapsule with 92% cell density. a cell density of 5.5 x 107 cells/ml microcapsule with >85% cell density was achieved within microcapsules with 15 m membrane thickness and these microcapsules kept over 88% integrity ratio after 11 days, which was much higher than that of microcapsules with 10 m membrane thickness. membrane with more than 20 m thickness was not suited for encapsulated cell culture owing to low-protein diffusion rate. these results indicated that cells survived shortly within the thinnest membrane thickness. there was a specific membrane thickness more suitable for cell growth for a long-time culture. these findings will be useful for preparing microcapsules with the desired membrane thickness for microencapsulated cell culture dependent on various purposes. (c) 2012 wiley periodicals, inc. j biomed mater res part a, 2013. |
| WOS标题词 | science & technology ; technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | engineering, biomedical ; materials science, biomaterials |
| 研究领域[WOS] | engineering ; materials science |
| 关键词[WOS] | alginate polycation microcapsules ; l-lysine microcapsules ; microencapsulation ; optimization ; growth ; tumor ; endostatin ; stability ; viability ; delivery |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315406300011 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117670]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dept Biotechnol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Liaoning Entry Exit Inspect & Quarantine Bur, Dalian 116001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Ying,Zhang, Ying,Wang, Yu,et al. Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation[J]. journal of biomedical materials research part a,2013,101(4):1007-1015. |
| APA | Ma, Ying.,Zhang, Ying.,Wang, Yu.,Wang, Qiuyan.,Tan, Mingqian.,...&Ma, Xiaojun.(2013).Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation.journal of biomedical materials research part a,101(4),1007-1015. |
| MLA | Ma, Ying,et al."Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation".journal of biomedical materials research part a 101.4(2013):1007-1015. |
入库方式： OAI收割
来源：大连化学物理研究所
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