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The quantitative proteomic analysis reveals schisantherin a prevents liver fibrosis through regulating extracellular matrix organization

文献类型：期刊论文

作者Lu, Qi3,4; Huang, Hui3; Liu, Qian3; Wang, Yuqiu3; Meng, Qian3; Fang, Shanhua3; Liu, Ping1,2; Zhou, Hu3,4
刊名INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
出版日期2022-10-01
卷号480页码:8
ISSN号1387-3806
DOI10.1016/j.ijms.2022.116898
通讯作者Fang, Shanhua(shanhua125@163.com) ; Liu, Ping(liuliver@vip.sina.com) ; Zhou, Hu(zhouhu@simm.ac.cn)
英文摘要The activation of hepatic stellate cell (HSC) induced by transforming growth factor-01 (TGF-01) is the key event in the pathogenesis of liver fibrosis. Schisantherin A (SCA), a main active ingredient of Schisandra chinensis, has anti-tumor and anti-inflammatory activities. Here, we reported that SCA inhibited the activation, proliferation, and cell cycle of human HSC cell line LX2. Then, by performing the tandem mass tag (TMT)-based quantitative proteomic analysis on LX2, we identified a total of 6045 proteins across the control, TGF-01-activated and SCA treated LX2 groups, of which 544 proteins were significantly changed among the three groups. All the differentially expressed proteins (DEPs) were assigned to 4 clusters by fuzzy c-means (FCM) clustering analysis. The changed expression of DEPs in cluster 3 and 4 was reversed by SCA treatment. Bioinformatic analysis revealed that SCA regulated the expression of several DEPs involved in extracellular matrix organization, such as thrombospondin 1 (THBS1), transgelin (TAGLN) and tissue inhibitor of metalloproteinase 3 (TIMP3). The Western blot and RT-qPCR analysis confirmed these proteins increased in TGF-01 group and decreased by the SCA treatment. In summary, we found that SCA may exert anti-fibrotic effect on HSCs by regulating the process of extracellular matrix organization in HSCs. Keywords: liver fibrosis, schisantherin A, proteomics, extracellular matrix organization. (C) 2022 Elsevier B.V. All rights reserved.
WOS关键词MAXQUANT COMPUTATIONAL PLATFORM ; TARGET
资助项目Shanghai Municipal Science and Technology Committee of Shanghai outstanding academic leaders plan[20XD1424900] ; National Natural Science Foundation of China[81530101]
WOS研究方向Physics ; Spectroscopy
语种英语
WOS记录号WOS:000855068800005
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/302471]  
专题新药研究国家重点实验室
通讯作者Fang, Shanhua; Liu, Ping; Zhou, Hu
作者单位1.Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Inst Liver Dis, Key Lab Liver & Kidney Dis,Minist Educ,Dept Pharm, 528 Zhangheng Rd, Shanghai 201203, Peoples R China
2.Shanghai Univ Tradit Chinese Med, E Inst Shanghai Municipal Educ Comm, 1200 Cailun Rd, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, State Key Lab Drug Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China
4.Nanjing Univ Chinese Med, Sch Chinese Mat Med, 138 Xianlin Ave, Nanjing 210023, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714		 Lu, Qi,Huang, Hui,Liu, Qian,et al. The quantitative proteomic analysis reveals schisantherin a prevents liver fibrosis through regulating extracellular matrix organization[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2022,480:8.
APA Lu, Qi.,Huang, Hui.,Liu, Qian.,Wang, Yuqiu.,Meng, Qian.,...&Zhou, Hu.(2022).The quantitative proteomic analysis reveals schisantherin a prevents liver fibrosis through regulating extracellular matrix organization.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,480,8.
MLA Lu, Qi,et al."The quantitative proteomic analysis reveals schisantherin a prevents liver fibrosis through regulating extracellular matrix organization".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 480(2022):8.

入库方式： OAI收割

来源：上海药物研究所

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