Determination of six core components from Mahuang Xuanfei Zhike syrup in rat plasma and tissues by UPLC-MS/MS: Application to a pharmacokinetics and tissue distribution study
文献类型：期刊论文
| 作者 | Yang, Yang3; Zhu, Jing2; Yao, Chang-liang1; Guo, De-an1 ; He, Na2; Mei, Quan-xi3; Feng, Guang-jun3; Chen, Qin-hua3; Yang, Guang-yi2
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| 刊名 | BIOMEDICAL CHROMATOGRAPHY
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| 出版日期 | 2022-09-21 |
| 页码 | 15 |
| ISSN号 | 0269-3879 |
| 关键词 | Mahuang Xuanfei Zhike syrup pharmacokinetics tissue distribution UPLC-MS MS |
| DOI | 10.1002/bmc.5496 |
| 通讯作者 | Chen, Qin-hua(cqh77@163.com) ; Yang, Guang-yi(13971908298@163.com) |
| 英文摘要 | Mahuang Xuanfei Zhike (MXZ) syrup, a Chinese patent medicine, has been widely used in the clinical treatment of cough. However, there is no reported method for the quantitative analysis of the effective components of MXZ syrup in biological samples. In this study, the effective components of MXZ syrup were screened by network pharmacology and molecular docking technology. A sensitive and rapid ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to test the active components of MXZ syrup in rat plasma and tissue homogenates, including ephedrine, amygdalin, chlorogenic acid, harpagoside, forsythin and forsythoside A. Chromatographic separation was performed on a Waters Acquity UPLC HSS T3 column (2.1 x 50 mm, 1.8 mu m) and the mass analysis was conducted using a Waters Xevo TQ mass spectrometer using multiple reaction positive and negative ion simultaneous monitoring mode. The results showed that the linearity ranged from 0.3 to 409.4 ng/ml. The extraction recoveries were all <8.33%, and the matrix effects were all <8.45, which met the requirements. The pharmacokinetic and tissue distribution results indicated that the main active components of MXZ syrup were absorbed quickly and eliminated slowly in vivo, and there may be a reabsorption process. |
| WOS关键词 | NETWORK PHARMACOLOGY ; MECHANISMS ; AMYGDALIN |
| 资助项目 | Sanming Project of Medicine in Shenzhen[SZZYSM202106004] ; Guangdong Provincial Hospital Pharmacy Research Fund (Australia Fund)[2022A41] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000858230100001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/302486]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chen, Qin-hua; Yang, Guang-yi |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 2.Guangzhou Univ Chinese Med, Shenzhen Baoan Tradit Chinese Med Hosp, Shenzhen 518000, Guangdong, Peoples R China 3.Shenzhen Baoan Authent TCM Therapy Hosp, Key Lab TCM Clin Pharm, Shenzhen 518101, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yang,Zhu, Jing,Yao, Chang-liang,et al. Determination of six core components from Mahuang Xuanfei Zhike syrup in rat plasma and tissues by UPLC-MS/MS: Application to a pharmacokinetics and tissue distribution study[J]. BIOMEDICAL CHROMATOGRAPHY,2022:15. |
| APA | Yang, Yang.,Zhu, Jing.,Yao, Chang-liang.,Guo, De-an.,He, Na.,...&Yang, Guang-yi.(2022).Determination of six core components from Mahuang Xuanfei Zhike syrup in rat plasma and tissues by UPLC-MS/MS: Application to a pharmacokinetics and tissue distribution study.BIOMEDICAL CHROMATOGRAPHY,15. |
| MLA | Yang, Yang,et al."Determination of six core components from Mahuang Xuanfei Zhike syrup in rat plasma and tissues by UPLC-MS/MS: Application to a pharmacokinetics and tissue distribution study".BIOMEDICAL CHROMATOGRAPHY (2022):15. |
入库方式： OAI收割
来源：上海药物研究所
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