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Current Analytical Strategies for Antibody-Drug Conjugates in Biomatrices

文献类型：期刊论文

作者Qin, Qiuping3,4; Gong, Likun1,2,3,4
刊名MOLECULES
出版日期2022-10-01
卷号27期号:19页码:17
关键词ADC antibody payload LBA LC-MS bioanalysis immunogenicity interference
DOI10.3390/molecules27196299
通讯作者Qin, Qiuping(qpqin@cdser.simm.ac.cn) ; Gong, Likun(lkgong@cdser.simm.ac.cn)
英文摘要Antibody-drug conjugates (ADCs) are a new class of biotherapeutics, consisting of a cytotoxic payload covalently bound to an antibody by a linker. Ligand-binding assay (LBA) and liquid chromatography-mass spectrometry (LC-MS) are the favored techniques for the analysis of ADCs in biomatrices. The goal of our review is to provide current strategies related to a series of bioanalytical assays for pharmacokinetics (PK) and anti-drug antibody (ADA) assessments. Furthermore, the strengths and limitations of LBA and LC-MS platforms are compared. Finally, potential factors that affect the performance of the developed assays are also provided. It is hoped that the review can provide valuable insights to bioanalytical scientists on the use of an integrated analytical strategy involving LBA and LC-MS for the bioanalysis of ADCs and related immunogenicity evaluation.
WOS关键词LC-MS/MS METHOD ; THERAPEUTIC PROTEINS ; TARGET INTERFERENCE ; NEUTRALIZING ANTIBODY ; IMMUNOGENICITY ASSAYS ; BIOANALYTICAL METHODS ; SERUM SAMPLES ; IMMUNOASSAY ; VALIDATION ; TOLERANCE
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[No.XDA12050305] ; Strategic Priority Research Program of the Chinese Academy of Sciences[2021B0909050003] ; Department of Science and Technology of Guangdong Province
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000867011700001
出版者MDPI
源URL[http://119.78.100.183/handle/2S10ELR8/302781]  
专题新药研究国家重点实验室
通讯作者Qin, Qiuping; Gong, Likun
作者单位1.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China
2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China
3.Chinese Acad Sci, Ctr Drug Safety Evaluat & Res, Shanghai Inst Mat Med, Dept Immunoassay & Immunochem, Shanghai 201203, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Qin, Qiuping,Gong, Likun. Current Analytical Strategies for Antibody-Drug Conjugates in Biomatrices[J]. MOLECULES,2022,27(19):17.
APA Qin, Qiuping,&Gong, Likun.(2022).Current Analytical Strategies for Antibody-Drug Conjugates in Biomatrices.MOLECULES,27(19),17.
MLA Qin, Qiuping,et al."Current Analytical Strategies for Antibody-Drug Conjugates in Biomatrices".MOLECULES 27.19(2022):17.

入库方式： OAI收割

来源：上海药物研究所

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