A comparison study on the interactions of two oligosaccharides with tobacco cells by time-resolved fluorometric method
文献类型:期刊论文
作者 | Guo, Weihua2; Yin, Heng3; Ye, Zhiqiang1; Zhao, Xiaoming3; Yuan, Jingli1; Du, Yuguang3 |
刊名 | carbohydrate polymers
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出版日期 | 2012-09-01 |
卷号 | 90期号:1页码:491-495 |
关键词 | Oligosaccharide Tobacco cell Interaction Europium complex Time-resolved fluorescence |
产权排序 | 2,2 |
通讯作者 | jingliyuan ; 杜昱光 |
英文摘要 | the interactions of tobacco cells with two oceanic oligosaccharides, chitosan oligosaccharide (cos) and alginate derived oligosaccharide (aos) were investigated and compared by time-resolved fluorometric method using two eu3+ complexes as luminescence probes. the binding processes of two oligosaccharides and tobacco leaf cells (epidermal and stomatal guard cells) were characterized by the luminescence imaging method based on the reaction of tobacco cells with oligosaccharide (cos or aos) conjugated to a highly fluorescent eu3+ complex. in addition, the concentration changes of indole-3-acetic acid (iaa) and iaa-related peroxidase produced in the cells during the interaction of oligosaccharides (cos or aos) and tobacco cells were also determined. the results indicate that the bioactivity of cos, with faster binding rate to the tobacco cells and stronger effect on the iaa generation in the cells, is remarkably higher than that of aos. the comparison study on bioactivities of the two oligosaccharides reveals the relationship between structures and bioactivities of oligosaccharide, and suggests that cos should be more favorable to be used as a promotion reagent of plant growth in agriculture field. (c) 2012 elsevier ltd. all rights reserved. |
WOS标题词 | science & technology ; physical sciences |
学科主题 | 物理化学 |
类目[WOS] | chemistry, applied ; chemistry, organic ; polymer science |
研究领域[WOS] | chemistry ; polymer science |
关键词[WOS] | oligochitosan ; plants ; cos |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000308267000067 |
公开日期 | 2013-10-11 |
源URL | [http://159.226.238.44/handle/321008/117748] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Dalian Univ Technol, Dept Chem, State Key Lab Fine Chem, Dalian 116024, Peoples R China 2.Dalian Ocean Univ, Sch Life Sci & Technol, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China |
推荐引用方式 GB/T 7714 | Guo, Weihua,Yin, Heng,Ye, Zhiqiang,et al. A comparison study on the interactions of two oligosaccharides with tobacco cells by time-resolved fluorometric method[J]. carbohydrate polymers,2012,90(1):491-495. |
APA | Guo, Weihua,Yin, Heng,Ye, Zhiqiang,Zhao, Xiaoming,Yuan, Jingli,&Du, Yuguang.(2012).A comparison study on the interactions of two oligosaccharides with tobacco cells by time-resolved fluorometric method.carbohydrate polymers,90(1),491-495. |
MLA | Guo, Weihua,et al."A comparison study on the interactions of two oligosaccharides with tobacco cells by time-resolved fluorometric method".carbohydrate polymers 90.1(2012):491-495. |
入库方式: OAI收割
来源:大连化学物理研究所
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