中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A comparison study on the interactions of two oligosaccharides with tobacco cells by time-resolved fluorometric method

文献类型:期刊论文

作者Guo, Weihua2; Yin, Heng3; Ye, Zhiqiang1; Zhao, Xiaoming3; Yuan, Jingli1; Du, Yuguang3
刊名carbohydrate polymers
出版日期2012-09-01
卷号90期号:1页码:491-495
关键词Oligosaccharide Tobacco cell Interaction Europium complex Time-resolved fluorescence
产权排序2,2
通讯作者jingliyuan ; 杜昱光
英文摘要the interactions of tobacco cells with two oceanic oligosaccharides, chitosan oligosaccharide (cos) and alginate derived oligosaccharide (aos) were investigated and compared by time-resolved fluorometric method using two eu3+ complexes as luminescence probes. the binding processes of two oligosaccharides and tobacco leaf cells (epidermal and stomatal guard cells) were characterized by the luminescence imaging method based on the reaction of tobacco cells with oligosaccharide (cos or aos) conjugated to a highly fluorescent eu3+ complex. in addition, the concentration changes of indole-3-acetic acid (iaa) and iaa-related peroxidase produced in the cells during the interaction of oligosaccharides (cos or aos) and tobacco cells were also determined. the results indicate that the bioactivity of cos, with faster binding rate to the tobacco cells and stronger effect on the iaa generation in the cells, is remarkably higher than that of aos. the comparison study on bioactivities of the two oligosaccharides reveals the relationship between structures and bioactivities of oligosaccharide, and suggests that cos should be more favorable to be used as a promotion reagent of plant growth in agriculture field. (c) 2012 elsevier ltd. all rights reserved.
WOS标题词science & technology ; physical sciences
学科主题物理化学
类目[WOS]chemistry, applied ; chemistry, organic ; polymer science
研究领域[WOS]chemistry ; polymer science
关键词[WOS]oligochitosan ; plants ; cos
收录类别SCI
语种英语
WOS记录号WOS:000308267000067
公开日期2013-10-11
源URL[http://159.226.238.44/handle/321008/117748]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, Dept Chem, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Dalian Ocean Univ, Sch Life Sci & Technol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Guo, Weihua,Yin, Heng,Ye, Zhiqiang,et al. A comparison study on the interactions of two oligosaccharides with tobacco cells by time-resolved fluorometric method[J]. carbohydrate polymers,2012,90(1):491-495.
APA Guo, Weihua,Yin, Heng,Ye, Zhiqiang,Zhao, Xiaoming,Yuan, Jingli,&Du, Yuguang.(2012).A comparison study on the interactions of two oligosaccharides with tobacco cells by time-resolved fluorometric method.carbohydrate polymers,90(1),491-495.
MLA Guo, Weihua,et al."A comparison study on the interactions of two oligosaccharides with tobacco cells by time-resolved fluorometric method".carbohydrate polymers 90.1(2012):491-495.

入库方式: OAI收割

来源:大连化学物理研究所

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