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The Detection of Divalent Iron and Reactive Oxygen Species During Ferroptosis with the Use of a Dual-Reaction Turn-On Fluorescent Probe

文献类型：期刊论文

作者Wang, Yueqi2,3; Li, Changjian1,4; Zhuo, Jiaming2; Hui, Hui2,3; Zhou, Bing1; Tian, Jie1,2,3,4,5
刊名MOLECULAR IMAGING AND BIOLOGY
出版日期2022-10-04
页码12
ISSN号1536-1632
关键词Dual-reaction Fe(II) Ferroptosis Fluorescent probes ROS Turn-on response
DOI10.1007/s11307-022-01774-6
通讯作者Hui, Hui(hui.hui@ia.ac.cn) ; Zhou, Bing(bingzh@buaa.edu.cn) ; Tian, Jie(tian@ieee.org)
英文摘要Purpose Ferroptosis, a programmed cell death modality, is an iron-dependent, non-apoptosis pathway that is characterized by the upregulation of divalent iron and reactive oxygen species (ROS) levels. However, the sensitive and rapid detection to track changes in ferroptosis is challenging, partially due to the lack of methods for monitoring the Fe(II) accumulation and ROS generation. Procedures Herein, we reported a dual-reaction fluorescent probe DR-1 with turn-on response, which realized the simultaneous visualizing of Fe(II) and ROS with a single probe. The structure of fluorescence quenching group and turn-on fluorophore constitute a double switch for DR-1, which increases its specificity and stability. Results During ferroptotic cell death, the upregulation of ROS levels led to the cleavage of quenching group of DR-1, and the aggregation of Fe(II) resulting in fluorescence recovery. Conclusions Overall, this study provides a new dual-reaction probe that shows the great potential to explore the mechanism of ferroptosis in vitro and in vivo by fluorescence imaging.
WOS关键词LABILE IRON ; CELL-DEATH ; LIPID-PEROXIDATION ; CANCER-CELLS ; GPX4 ; DYSFUNCTION ; EXPRESSION ; FE(II) ; STATE
资助项目National Key Research and Development Program of China[2017YFA0700401] ; National Key Research and Development Program of China[2016YFC0103803] ; National Key Research and Development Program of China[2017YFA0205200] ; National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[81930053] ; National Natural Science Foundation of China[81827808] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81971198] ; National Natural Science Foundation of China[81671851] ; China Postdoctoral Science Foundation[2020M680301] ; China Postdoctoral Science Foundation[2019TQ0018] ; Chinese Academy of Sciences Youth Innovation Promotion Association[2018167] ; Chinese Academy of Sciences Key Technology Talent Program ; Project of High-Level Talents Team Introduction in Zhuhai City[Zhuhai HLHPTP201703] ; Fundamental Research Funds for the Central Universities[JKF-YG-22-B005]
WOS研究方向Radiology, Nuclear Medicine & Medical Imaging
语种英语
出版者SPRINGER
WOS记录号WOS:000863801600001
资助机构National Key Research and Development Program of China ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; Chinese Academy of Sciences Youth Innovation Promotion Association ; Chinese Academy of Sciences Key Technology Talent Program ; Project of High-Level Talents Team Introduction in Zhuhai City ; Fundamental Research Funds for the Central Universities
源URL[http://ir.ia.ac.cn/handle/173211/50367]  
专题自动化研究所_中国科学院分子影像重点实验室
通讯作者Hui, Hui; Zhou, Bing; Tian, Jie
作者单位1.Beihang Univ, Sch Engn Med, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Automat, Beijing Key Lab Mol Imaging, CAS Key Lab Mol Imaging,State Key Lab Management, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Beihang Univ, Minist Ind & Informat Technol, Key Lab Big Data Based Precis Med, Beijing 100191, Peoples R China
5.Jinan Univ, Zhuhai Peoples Hosp, Zhuhai Precis Med Ctr, Zhuhai 519000, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yueqi,Li, Changjian,Zhuo, Jiaming,et al. The Detection of Divalent Iron and Reactive Oxygen Species During Ferroptosis with the Use of a Dual-Reaction Turn-On Fluorescent Probe[J]. MOLECULAR IMAGING AND BIOLOGY,2022:12.
APA Wang, Yueqi,Li, Changjian,Zhuo, Jiaming,Hui, Hui,Zhou, Bing,&Tian, Jie.(2022).The Detection of Divalent Iron and Reactive Oxygen Species During Ferroptosis with the Use of a Dual-Reaction Turn-On Fluorescent Probe.MOLECULAR IMAGING AND BIOLOGY,12.
MLA Wang, Yueqi,et al."The Detection of Divalent Iron and Reactive Oxygen Species During Ferroptosis with the Use of a Dual-Reaction Turn-On Fluorescent Probe".MOLECULAR IMAGING AND BIOLOGY (2022):12.

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