Fusion and expansion of vitellogenin vesicles during Caenorhabditis elegans intestinal senescence
文献类型：期刊论文
| 作者 | Zhai, Chao5,6; Zhang, Nan5 ; Li, Xi-Xia4; Chen, Xi1; Sun, Fei2,3,4; Dong, Meng-Qiu5
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| 刊名 | AGING CELL
 |
| 出版日期 | 2022-10-05 |
| 页码 | 13 |
| ISSN号 | 1474-9718 |
| 关键词 | Aging C elegans Senescence Vesicle fusion Vitellogenin Yolk |
| DOI | 10.1111/acel.13719 |
| 通讯作者 | Sun, Fei(feisun@ibp.ac.cn) ; Dong, Meng-Qiu(dongmengqiu@nibs.ac.cn) |
| 英文摘要 | Some of the most conspicuous aging phenotypes of C. elegans are related to post-reproductive production of vitellogenins (Vtg), which form yolk protein (YP) complexes after processing and lipid loading. Vtg/YP levels show huge increases with age, and inhibition of this extends lifespan, but how subcellular and organism-wide distribution of these proteins changes with age has not been systematically explored. Here, this has been done to understand how vitellogenesis promotes aging. The age-associated changes of intestinal vitellogenin vesicles (VVs), pseudocoelomic yolk patches (PYPs), and gonadal yolk organelles (YOs) have been characterized by immuno-electron microscopy. We find that from reproductive adult day 2 (AD 2) to post-reproductive AD 6 and AD 9, intestinal VVs expand from 0.2 to 3-4 mu m in diameter or by >3000 times in volume, PYPs increase by >3 times in YP concentration and volume, while YOs in oocytes shrink slightly from 0.5 to 0.4 mu m in diameter or by 49% in volume. In AD 6 and AD 9 worms, mislocalized YOs found in the hypodermis, uterine cells, and the somatic gonadal sheath can reach a size of 10 mu m across in the former two tissues. This remarkable size increase of VVs and that of mislocalized YOs in post-reproductive worms are accompanied by extensive fusion between these Vtg/YP-containing vesicular structures in somatic cells. In contrast, no fusion is seen between YOs in oocytes. We propose that in addition to the continued production of Vtg, excessive fusion between VVs and mislocalized YOs in the soma worsen the aging pathologies seen in C. elegans. |
| WOS关键词 | YOLK PROTEINS ; LONGEVITY |
| 资助项目 | Beijing Municipal Science and Technology Commission ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China[31501160] ; National Natural Science Foundation of China[31925026] ; National Natural Science Foundation of China[32061143020] ; Institute of Chemistry, Chinese Academy of Sciences ; National Institutes of Health |
| WOS研究方向 | Cell Biology ; Geriatrics & Gerontology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000864029100001 |
| 资助机构 | Beijing Municipal Science and Technology Commission ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China ; Institute of Chemistry, Chinese Academy of Sciences ; National Institutes of Health |
| 源URL | [http://ir.ia.ac.cn/handle/173211/50397]  |
| 专题 | 类脑智能研究中心_微观重建与智能分析 |
| 通讯作者 | Sun, Fei; Dong, Meng-Qiu |
| 作者单位 | 1.Chinese Acad Sci, Inst Automat, Beijing, Peoples R China 2.Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Natl Key Lab Biomacromol, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Biol Imaging, Inst Biophys, Beijing, Peoples R China 5.Natl Inst Biol Sci, Beijing, Peoples R China 6.Peking Univ, Sch Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhai, Chao,Zhang, Nan,Li, Xi-Xia,et al. Fusion and expansion of vitellogenin vesicles during Caenorhabditis elegans intestinal senescence[J]. AGING CELL,2022:13. |
| APA | Zhai, Chao,Zhang, Nan,Li, Xi-Xia,Chen, Xi,Sun, Fei,&Dong, Meng-Qiu.(2022).Fusion and expansion of vitellogenin vesicles during Caenorhabditis elegans intestinal senescence.AGING CELL,13. |
| MLA | Zhai, Chao,et al."Fusion and expansion of vitellogenin vesicles during Caenorhabditis elegans intestinal senescence".AGING CELL (2022):13. |
入库方式： OAI收割
来源：自动化研究所
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