Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip
文献类型：会议论文
| 作者 | Huang,Chenghong; Tang,Hong; Chen,Hongjie; Huang,Xiaorong; Fang,Ying; Wang,KangRui; Men,Rongrong; Gao,Jie; Wang,Yang; Chen YY(陈艳艳)
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| 出版日期 | 2022 |
| 会议日期 | OCT 22-25, 2021 |
| 会议地点 | Xian, PEOPLES R CHINA |
| 关键词 | protein chip alpha-fetoprotein site-directed immobilization of antibody |
| 页码 | 148-155 |
| 英文摘要 | For immunoassay, immobilization of antibody is the most important technique in bioengineering for maintaining its intrinsic activity of antigen binding. The sugar chain in the Fc region carried by antibody can be oxidized into aldehyde group by sodium m-periodate, which can couple with the amino-group on chemistry modified substrate surface through covalent linking. Thereby, in situ site-directed immobilization of the oxidized antibody for only one-step reaction can be performed. Here we propose a facile method that oxidized anti-human alpha-fetoprotein antibody (anti-AFP) was in situ site-directed immobilized on APTES treated silicon substrate utilizing microfluidic channel system for AFP immunoassay. The shape of the immobilized anti-AFP was observed by AFM and imaged by imaging ellipsometry of self-developed protein system. Results shown that the amount of antibody immobilization amount and capacity of antigen binding of random immobilization and site-directed immobilization were separately enhanced 16.0% and 7.0%. the suggested strategy has the advantages of simple, environment-friend and convenient-operation. |
| 资助机构 | NSF of Chongqing [cstc2019jscx-msxm0366]; Open Fund of Chongqing Key Laboratory of Industrial Fermentation Microorganism [GYFJWSW-201905]; Project of Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJZD-K201806401, KJZD-K201906401]; graduate Innovation Fund of CQUST [YKJCX2020509, YKJCX2120502, YKJCX2120541, 202111551006] |
| 会议录 | INTEGRATED FERROELECTRICS
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| 语种 | 英语 |
| URL标识 | 查看原文 |
| WOS记录号 | WOS:000805668600015 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/90524]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Mech, Beijing, Peoples R China 2.Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China 3.CQUST, Chongqing Key Lab Ind Fermentat Microorganism, Chongqing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang,Chenghong,Tang,Hong,Chen,Hongjie,et al. Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip[C]. 见:. Xian, PEOPLES R CHINA. OCT 22-25, 2021. |
入库方式： OAI收割
来源：力学研究所
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