Large-scale preparation of two minor polar polyphenols from Phyllanthus emblica Linn. by macroporous resin column chromatography and high-temperature preparative high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Song, ZB; Chen, T; Wang, S; Shen, C; Li, HM; Li, AJ; Li, PP; Ma, YM; Chen, Z; Li, YL
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
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| 出版日期 | 2022 |
| 卷号 | 45期号:21页码:3923 |
| 英文摘要 | In the present study, an efficient strategy for the large-scale preparation of two minor polar polyphenols from Phyllanthus emblica L. has been established by macroporous resin column chromatography and high-temperature preparative high-speed counter-current chromatography. Macroporous resin column chromatography was used for the enrichment of the target polyphenols. The target polyphenols could be well enriched in the 10% ethanol fraction and the peak area proportion increased from 2.2% in the crude extract to 85.3% in the 10% ethanol fraction. Then, the 10% ethanol fraction was introduced to preparative high-speed counter-current chromatography for separation using an n-butanol-ethyl acetate water (17:3:20, v/v/v) solvent system. In order to improve the retention of the stationary phase, a high-temperature preparative high-speed counter-current chromatography had been developed by using a 316 L stainless steel pipe as a column. A 50 degrees C of column temperature was used and the retention rate of the stationary phase could reach 75%. Finally; 871 mg of compound I and 534 mg of compound H with purities higher than 98% were obtained from 4 g of the sample. The chemical structures were identified as corilagin and 1, 6-di-O-galloyl-beta-D-glucoside by H-1-NMR and C-13-NMR. |
| 源URL | [http://210.75.249.4/handle/363003/61077]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Song, ZB,Chen, T,Wang, S,et al. Large-scale preparation of two minor polar polyphenols from Phyllanthus emblica Linn. by macroporous resin column chromatography and high-temperature preparative high-speed counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2022,45(21):3923. |
| APA | Song, ZB.,Chen, T.,Wang, S.,Shen, C.,Li, HM.,...&Li, YL.(2022).Large-scale preparation of two minor polar polyphenols from Phyllanthus emblica Linn. by macroporous resin column chromatography and high-temperature preparative high-speed counter-current chromatography.JOURNAL OF SEPARATION SCIENCE,45(21),3923. |
| MLA | Song, ZB,et al."Large-scale preparation of two minor polar polyphenols from Phyllanthus emblica Linn. by macroporous resin column chromatography and high-temperature preparative high-speed counter-current chromatography".JOURNAL OF SEPARATION SCIENCE 45.21(2022):3923. |
入库方式： OAI收割
来源：西北高原生物研究所
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