Mechanism of Early Flowering in a Landrace Naked Barley eam8.l Mutant
文献类型：期刊论文
| 作者 | Mao, CZ; Xu, JQ; Wang, HD ; Chen, TR; Bian, HY; You, E; Shen, YH; Wang, L
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| 刊名 | AGRONOMY-BASEL
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| 出版日期 | 2022 |
| 卷号 | 12期号:9 |
| 英文摘要 | The EARLY MATURITY 8 (EAM8) gene of barley is homologous to the EARLY FLOWERING 3 (ELF3) gene in Arabidopsis, as loss-of-function mutations in this circadian clock gene promote rapid flowering. A previous study demonstrated that the early flowering phenotype of a hulless barley, Lalu, was due to allele eam8.l carrying an alternative splicing mutation in intron 3 that led to intron retention. In the present study, we verified that eam8.l encoded a truncated protein. Although EAM8 was expressed at a higher level in Lalu than in other barley lines with a longer growth period, it did not negatively regulate flowering time. This result further proved that the eam8.l protein was nonfunctional in regulating flowering in barley. The early flowering phenotype of Lalu plants was strongly dependent on the biosynthesis of gibberellin (GA). The eam8.l mutation stopped the suppression of GA biosynthesis, and Lalu accumulated excessive GA, especially in leaves. This was achieved through the upregulated expression of genes in the GA pathway, including GA20ox2, LFY1, SOC1, PAP2, and FPF3. The mutation of the EAM8 gene also abolished the inhibition of FLOWERING LOCUS T-like (FT1) gene expression at night. During the night, expression levels of the FT1 gene were higher than those during the day in Lalu. However, the GA-dependent pathway and FT1 gene mechanism are two independent pathways that promote flowering in Lalu. Alleles of EAM8, therefore, demonstrated an important breeding value in barley, which is probably effective in many other day-length-sensitive crop plants as well; thus, they could be used to tune adaptation in different geographic regions and climatic conditions, a critical issue in times of global warming. |
| 源URL | [http://210.75.249.4/handle/363003/61118]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Mao, CZ,Xu, JQ,Wang, HD,et al. Mechanism of Early Flowering in a Landrace Naked Barley eam8.l Mutant[J]. AGRONOMY-BASEL,2022,12(9). |
| APA | Mao, CZ.,Xu, JQ.,Wang, HD.,Chen, TR.,Bian, HY.,...&Wang, L.(2022).Mechanism of Early Flowering in a Landrace Naked Barley eam8.l Mutant.AGRONOMY-BASEL,12(9). |
| MLA | Mao, CZ,et al."Mechanism of Early Flowering in a Landrace Naked Barley eam8.l Mutant".AGRONOMY-BASEL 12.9(2022). |
入库方式： OAI收割
来源：西北高原生物研究所
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