Novel Hina alleles created by genome editing increase grain hardness and reduce grain width in barley
文献类型：期刊论文
| 作者 | Jiang, YY; Li, JM; Liu, BL; Cao, D; Zong, Y; Chang, YZ; Li, Y
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| 刊名 | TRANSGENIC RESEARCH
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| 出版日期 | 2022 |
| 英文摘要 | The hordoindolina genes (Hina and Hinb) are believed to play critical roles in barley (Hordeum vulgare L.) grain texture. In this study, we created novel alleles of the Hina gene using CRISPR/Cas9 (Clustered regularly inter spaced short palindromic repeat-associated protein, CRISPR-Cas) genome editing. Mutagenesis of single bases in these novel alleles led to loss of Hina protein function in edited lines. The grain hardness index of hina mutants was 95.5 on average, while that of the wild type was only 53.7, indicating successful conversion of soft barley into hard barley. Observation of cross-sectional grain structure using scanning electron microscopy revealed different adhesion levels between starch granules and protein matrix. Starch granules were loose and separated from the protein matrix in the wild type, but deeply trapped and tightly integrated with the protein matrix in hina02 mutants. In addition, the grain width and thousand-grain weight of the hina02 mutant were significantly lower than those of the wild type. |
| 源URL | [http://210.75.249.4/handle/363003/61132]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Jiang, YY,Li, JM,Liu, BL,et al. Novel Hina alleles created by genome editing increase grain hardness and reduce grain width in barley[J]. TRANSGENIC RESEARCH,2022. |
| APA | Jiang, YY.,Li, JM.,Liu, BL.,Cao, D.,Zong, Y.,...&Li, Y.(2022).Novel Hina alleles created by genome editing increase grain hardness and reduce grain width in barley.TRANSGENIC RESEARCH. |
| MLA | Jiang, YY,et al."Novel Hina alleles created by genome editing increase grain hardness and reduce grain width in barley".TRANSGENIC RESEARCH (2022). |
入库方式： OAI收割
来源：西北高原生物研究所
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