Xanthotoxol from Saussurea obvallata Attenuates LPS-Induced RAW 264.7 Cells Inflammatory Responses through NF-kappa B Pathway
文献类型：期刊论文
| 作者 | Wang, WD; Zhang, J; Liu, ZG ; Zhu, YH; Mei, LJ; Tao, YD; Jiang, L
|
| 刊名 | RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
 |
| 出版日期 | 2022 |
| 卷号 | 48期号:2页码:300 |
| 英文摘要 | Saussurea obvallata has been used as a medicinal herb to treat rheumatoid arthritis in Qinghai-Tibet Plateau. Xanthotoxol, one of the major ingredients of S. obvallata, displays anti-inflammation bioactivities according to previous studies. However, the underlying mechanism of xanthotoxol in anti-inflammation have not yet been elucidated. In this study, XT was isolated from S. obvallata for the first time and we investigated the anti-inflammatory activity and the related molecular mechanism in LPS-activated macrophages in vitro model. The cytotoxicity of XT was determined through MTT assay and flow cytometry analysis. The anti-inflammatory activities of XT were determined according to its inhibitory effects on NO, and alpha pro-inflammatory cytokines. The date showed that XT significantly reduced inflammatory cytokines TNF-alpha, IL-6, and IL-1 beta in LPS-induced RAW264.7 cells as well as the synthesis of NO in a dose dependent manner. Moreover, xanthotoxol down-regulated the phosphorylation of NF-kappa B factors (p-P65, p-I kappa B alpha, p-IKK alpha/beta), the expressions of INOS and NFAT1 and the P65 translocation from the cytoplasm to the nucleus. These results suggested that XT exhibited significant anti-inflammatory activity in LPS-induced RAW264.7 cells model by inhibiting NF-kappa B pathway. These results might provide some explanations of the anti-inflammatory activity of S. obvallata and contribute to the further investigation and application of XT as a potential anti-inflammatory drug. |
| 源URL | [http://210.75.249.4/handle/363003/61255]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, WD,Zhang, J,Liu, ZG,et al. Xanthotoxol from Saussurea obvallata Attenuates LPS-Induced RAW 264.7 Cells Inflammatory Responses through NF-kappa B Pathway[J]. RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY,2022,48(2):300. |
| APA | Wang, WD.,Zhang, J.,Liu, ZG.,Zhu, YH.,Mei, LJ.,...&Jiang, L.(2022).Xanthotoxol from Saussurea obvallata Attenuates LPS-Induced RAW 264.7 Cells Inflammatory Responses through NF-kappa B Pathway.RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY,48(2),300. |
| MLA | Wang, WD,et al."Xanthotoxol from Saussurea obvallata Attenuates LPS-Induced RAW 264.7 Cells Inflammatory Responses through NF-kappa B Pathway".RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY 48.2(2022):300. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。