Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro
文献类型:期刊论文
作者 | Yuan, C; Dang, J; Han, Y; Liu, C; Yu, S; Lv, Y; Cui, YB; Wang, ZH; Li, G |
刊名 | RSC ADVANCES |
出版日期 | 2022 |
卷号 | 12期号:8页码:5031 |
英文摘要 | Dianthus superbus is a traditional Chinese medicine that is commonly utilized as a treatment for inflammation, pain, and immunological conditions. In this study, an anti-inflammatory maltol glycoside derived from Dianthus superbus was isolated for the first time via medium and high-pressure liquid chromatography, and at the same time, the in vitro anti-inflammatory activity of this maltol glycoside was preliminarily explored. Initially, crude samples of Dianthus superbus were preprocessed via MCI GEL (R) CHP20P and Spherical C18 medium-pressure chromatography, under the guidance of evaluation of in vitro anti-inflammatory activity. Fr44 was found to be the target fraction, and it was further isolated via two-dimensional reversed-phase/hydrophilic interaction liquid chromatography, yielding > 95% pure and was identified as tunicoside B. MTT assay, nitric oxide and nitric oxide synthase were used to evaluate the effects of tunicoside B on murine macrophage Raw264.7 by nitric oxide synthase assay kit, molecular docking, and western blotting. The results showed that tunicoside B did not affect the viability of cells and exhibited significant anti-inflammatory activity. As far as we know, this is the first report of tunicoside B from Dianthus superbus and the first study on the anti-inflammatory activity of tunicoside B. More importantly, the approach established in this study is expected to provide a theoretical basis for the separation and pharmacological activity study of maltol glycosides from other natural products. |
源URL | [http://210.75.249.4/handle/363003/61290] |
专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Yuan, C,Dang, J,Han, Y,et al. Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro[J]. RSC ADVANCES,2022,12(8):5031. |
APA | Yuan, C.,Dang, J.,Han, Y.,Liu, C.,Yu, S.,...&Li, G.(2022).Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro.RSC ADVANCES,12(8),5031. |
MLA | Yuan, C,et al."Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro".RSC ADVANCES 12.8(2022):5031. |
入库方式: OAI收割
来源:西北高原生物研究所
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