Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes
文献类型：期刊论文
| 作者 | Chen, T; Yang, X; Wang, S; Shen, C; Li, HM; Wei, YF; Yan, SP ; Song, ZB; Yang, FM; Liu, YR
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
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| 出版日期 | 2022 |
| 卷号 | 45期号:2页码:468 |
| 英文摘要 | The separation of compounds with similar polarities is challenging. In the present study, five flavone glycosides, including two groups with similar polarities, were obtained from Dracocephalum tanguticum by three high-speed counter-current chromatography modes, including flow rate conversion mode, recycling mode, and heart-cut mode. With flow rate conversion mode, compounds 3 and 4 with similar polarities and compound 5 were separated by high-speed counter-current chromatography with ethyl acetate/methanol/water (5.0% acetic acid) (8:2:10, v/v) system. The flow rate was controlled as: 1.8 mL/min for 0-160 min, 2.2 mL/min for 160-200 min, and 2.5 mL/min for 200-400 min. However, compounds 1 and 2 with similar polarities were not separated due to the similar distributive properties. Then, a recycling and heart-cut mode were introduced to improve the separation efficiency. The heart-cut mode was introduced in the second and third cycles, and compounds 1 and 2 were well separated in the fourth cycle. Consequently, five flavone glycosides, including two groups with similar polarities were obtained and identified as cosmosiin (1), pedaliin (2), quercetin-3-O-rutinoside (3), pedaliin-6''-acetate (4), and sorbifolin-6-O-beta-glucopyranoside (5). The current strategy provides a reference for separating compounds with similar polarities from a crude sample. |
| 源URL | [http://210.75.249.4/handle/363003/61345]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Chen, T,Yang, X,Wang, S,et al. Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes[J]. JOURNAL OF SEPARATION SCIENCE,2022,45(2):468. |
| APA | Chen, T.,Yang, X.,Wang, S.,Shen, C.,Li, HM.,...&Li, YL.(2022).Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes.JOURNAL OF SEPARATION SCIENCE,45(2),468. |
| MLA | Chen, T,et al."Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes".JOURNAL OF SEPARATION SCIENCE 45.2(2022):468. |
入库方式： OAI收割
来源：西北高原生物研究所
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