Molecular Karyotyping on Populus simonii x P. nigra and the Derived Doubled Haploid
文献类型：期刊论文
| 作者 | Liu, B; Wang, S; Tao, XY; Liu, CX; Qu, GZ; Dou, QW
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2021 |
| 卷号 | 22期号:21 |
| 英文摘要 | The molecular karyotype could represent the basic genetic make-up in a cell nucleus of an organism or species. A doubled haploid (DH) is a genotype formed from the chromosome doubling of haploid cells. In the present study, molecular karyotype analysis of the poplar hybrid Populus simonii x P. nigra (P. xiaohei) and the derived doubled haploids was carried out with labeled telomeres, rDNA, and two newly repetitive sequences as probes by fluorescence in situ hybridization (FISH). The tandem repeats, pPC349_XHY and pPD284_XHY, with high-sequence homology were used, and the results showed that they presented the colocalized distribution signal in chromosomes. For P. xiaohei, pPD284_XHY produced hybridizations in chromosomes 1, 5, 8, and 9 in the hybrid. The combination of pPD284_XHY, 45S rDNA, and 5S rDNA distinctly distinguished six pairs of chromosomes, and the three pairs of chromosomes showed a significant difference in the hybridization between homologous chromosomes. The repeat probes used produced similar FISH hybridizations in the DH; nevertheless, pPD284_XHY generated an additional hybridization site in the telomere region of chromosome 14. Moreover, two pairs of chromosomes showed differential hybridization distributions between homologous chromosomes. Comparisons of the distinguished chromosomes between hybrid and DH poplar showed that three pairs of chromosomes in the DH presented hybridization patterns that varied from those of the hybrid. The No. 8 chromosome in DH and one of the homologous chromosomes in P. xiaohei shared highly similar FISH patterns, which suggested the possibility of intact or mostly partial transfer of the chromosome between the hybrid and DH. Our study will contribute to understanding the genetic mechanism of chromosomal variation in P. xiaohei and derived DH plants. |
| 源URL | [http://210.75.249.4/handle/363003/61355]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Liu, B,Wang, S,Tao, XY,et al. Molecular Karyotyping on Populus simonii x P. nigra and the Derived Doubled Haploid[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(21). |
| APA | Liu, B,Wang, S,Tao, XY,Liu, CX,Qu, GZ,&Dou, QW.(2021).Molecular Karyotyping on Populus simonii x P. nigra and the Derived Doubled Haploid.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(21). |
| MLA | Liu, B,et al."Molecular Karyotyping on Populus simonii x P. nigra and the Derived Doubled Haploid".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.21(2021). |
入库方式： OAI收割
来源：西北高原生物研究所
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