Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry
文献类型：期刊论文
| 作者 | Zhang, Xihao1,2; He, Xiwen1,2; Chen, Langxing1,2; Zhang, Yukui1,2,3 |
| 刊名 | journal of materials chemistry
 |
| 出版日期 | 2012 |
| 卷号 | 22期号:32页码:16520-16526 |
| 产权排序 | 3,4 |
| 通讯作者 | 陈朗星 |
| 英文摘要 | in this study, a novel approach was developed to synthesize aminophenylboronic acid functionalized magnetic nanoparticles (nps) via cu(i)-catalyzed azide-alkyne cycloaddition (cuaac) 'click' chemistry. firstly, azide-functional fe3o4 nps were obtained by a two-step chemical modification process. then, an alkyne-phenylboronic acid molecule was connected onto the surface of magnetite by the cuaac reaction. the morphology, structure and composition of the synthesized nanocomposites were characterized by transmission electron microscopy (tem), x-ray powder diffraction (xrd), vibrating sample magnetometer (vsm), fourier transform infrared (ftir) spectroscopy and x-ray photoelectron spectrometry (xps). five proteins, including ovalbumin (ob), transferrin (trf), as glycoprotein templates and lysozyme (lyz), bovine serum albumin (bsa), horse heart cytochrome c (cyt c) as nonglycoprotein templates are chosen as target proteins. the as-prepared click-fe3o4@apba nps with a mean diameter of 23.2 nm showed a strong magnetic response to an externally applied magnetic field and exhibited a high adsorption capacity and excellent specificity towards glycoproteins in comparison with nonglycoproteins. the click-fe3o4@apba nps showed the higher adsorption capacity towards glycoproteins than the nonclick-fe3o4@apba nps which were synthesized through a common nucleophilic substitution reaction. the greatly enhanced adsorption capacity towards glycoproteins demonstrated that the 'click' method presented great superiority in ligand immobilization. finally, the click-fe3o4@apba nps could efficiently enrich glycoproteins from real egg white samples as well. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, physical ; materials science, multidisciplinary |
| 研究领域[WOS] | chemistry ; materials science |
| 关键词[WOS] | affinity monolithic column ; iron-oxide nanoparticles ; one-pot synthesis ; selective enrichment ; gold nanoparticles ; protein separation ; mesoporous silica ; facile synthesis ; glycopeptides ; glycosylation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000306708700051 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117833]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xihao,He, Xiwen,Chen, Langxing,et al. Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry[J]. journal of materials chemistry,2012,22(32):16520-16526. |
| APA | Zhang, Xihao,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2012).Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry.journal of materials chemistry,22(32),16520-16526. |
| MLA | Zhang, Xihao,et al."Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry".journal of materials chemistry 22.32(2012):16520-16526. |
入库方式： OAI收割
来源：大连化学物理研究所
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