Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and peptide separation for proteome analysis
文献类型：期刊论文
| 作者 | Xia, Simin1,2; Tao, Dingyin1,2; Yuan, Huiming1; Zhou, Yuan1,2; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2012-07-01 |
| 卷号 | 35期号:14页码:1764-1770 |
| 关键词 | Immobilized enzymatic reactor Multidimensional chromatography Nano-flow LC Online digestion Proteome |
| 产权排序 | 1,1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | an integrated multidimensional nano-flow liquid chromatography platform with the combination of protein and peptide separation via online digestion by an immobilized enzymatic reactor was established, and successfully applied for proteome analysis. by this platform, proteins were first separated by a weak anion and weak cation mixed-bed microcolumn under a series of salt steps, online digested by a trypsin immobilized enzymatic reactor, digests trapped and desalted by a c18 precolumn, separated by nano-reversed phase liquid chromatography, and finally identified by electrospray ionization-ms/ms. to evaluate the performance of such a platform, escherichia coli whole cell lysate proteins were analyzed. compared with the results obtained by shotgun approach, the identified protein number was increased by 6%, with the consumed time decreased from 38 to 14 h. we also compared with integrate platform based on micro-hplc, and the required sample amount was decreased to 8 mu g. these results demonstrated that such an integrated approach would be an attractive alternative to commonly applied approaches for proteome research. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | top-down proteomics ; monolith ; column |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000306493700009 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117846]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Simin,Tao, Dingyin,Yuan, Huiming,et al. Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and peptide separation for proteome analysis[J]. journal of separation science,2012,35(14):1764-1770. |
| APA | Xia, Simin.,Tao, Dingyin.,Yuan, Huiming.,Zhou, Yuan.,Liang, Zhen.,...&Zhang, Yukui.(2012).Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and peptide separation for proteome analysis.journal of separation science,35(14),1764-1770. |
| MLA | Xia, Simin,et al."Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and peptide separation for proteome analysis".journal of separation science 35.14(2012):1764-1770. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。