Hormone-responsive 3D multicellular culture model of human breast tissue
文献类型：期刊论文
| 作者 | Wang, Xiuli1,2; Kaplan, David L.1 |
| 刊名 | biomaterials
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 33期号:12页码:3411-3420 |
| 关键词 | 3D tissue Silk Breast Casein Epithelial cells Morphogenesis |
| 产权排序 | 2, 1 |
| 通讯作者 | davidlkaplan |
| 英文摘要 | a hormone-responsive 3d human tissue-like culture system was developed in which human primary mammary epithelial cells (mecs) were co-cultured with two types of predominant mammary stromal cells on silk protein scaffolds. silk porous scaffolds with incorporated extracellular matrix provided a compatible environment for epithelial structure morphogenesis and differentiation. the presence of stromal cells promoted mec proliferation, induced both alveolar and ductal morphogenesis and enhanced casein expression. in contrast, only alveolar structures were observed in monocultures. the alveolar structures generated from the heterotypic cultures in vitro exhibited proper polarity similar to human breast tissue in vivo. consistent with their phenotypic appearance, more functional differentiation of epithelial cells was also observed in the heterotypic cultures, where casein-alpha and -beta mrna expression were increased significantly. additionally, this 3d multicellular culture model displayed an estrogen-responsive physiologically relevant response, evidenced by enhanced cell proliferation, aberrant morphology, changes in gene expression profile and few polarized lumen structures after estrogen treatment. this culture system offers an excellent opportunity to explore the role of cell-cell and cell-substrate interactions during mammary gland development, the consequences of hormone receptor activation on mec behavior and morphogenesis, as well as their alteration during neoplastic transformation in human breast tissue. (c) 2012 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | engineering, biomedical ; materials science, biomaterials |
| 研究领域[WOS] | engineering ; materials science |
| 关键词[WOS] | mammary epithelial-cells ; hepatocyte growth-factor ; basement-membrane cultures ; mesenchymal stem-cells ; in-vitro ; alveolar morphogenesis ; tumor progression ; estrogen ; proliferation ; fibroblasts |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301886300004 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117862]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiuli,Kaplan, David L.. Hormone-responsive 3D multicellular culture model of human breast tissue[J]. biomaterials,2012,33(12):3411-3420. |
| APA | Wang, Xiuli,&Kaplan, David L..(2012).Hormone-responsive 3D multicellular culture model of human breast tissue.biomaterials,33(12),3411-3420. |
| MLA | Wang, Xiuli,et al."Hormone-responsive 3D multicellular culture model of human breast tissue".biomaterials 33.12(2012):3411-3420. |
入库方式： OAI收割
来源：大连化学物理研究所
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