Highly-efficient colony PCR method for red yeasts and its application to identify mutations within two leucine auxotroph mutants
文献类型：期刊论文
| 作者 | Lin, Xinping1,3; Yang, Fan1,2; Zhou, Yongjin1,3; Zhu, Zhiwei1,3; Jin, Guojie1,3; Zhang, Sufang1; Zhao, Zongbao Kent1 |
| 刊名 | yeast
 |
| 出版日期 | 2012-11-01 |
| 卷号 | 29期号:11页码:467-474 |
| 关键词 | red yeast screening colony PCR leucine auxotroph |
| 产权排序 | 1,1 |
| 通讯作者 | 张素芳 |
| 英文摘要 | red yeasts hold great promise in the production of microbial lipids and carotenoids. genetic study of red yeasts has attracted much attention; however, rapid amplification of genes from red yeast samples remains technically challenging. here a highly efficient method for the preparation of genomic dna (gdna) template, which could be directly used for pcr, was developed. cells from colonies or liquid cultures were collected and sequentially treated by microwave, plman5c, proteinase k and boiling (mmpb) in a single tube to give cell lysates that were qualified as pcr templates. single-copied gdna fragments o up to 2.8?kb were successfully amplified. we also demonstrated successful application of this method for species in the ascomycetes and basidiomycetes and identification of two leucine auxotroph mutants of rhodotorula glutinis. this method could be widely employed for the screening and genetic engineering of various yeasts. copyright (c) 2012 john wiley & sons, ltd. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; microbiology ; mycology |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; microbiology ; mycology |
| 关键词[WOS] | wall lytic enzyme ; rhodosporidium-toruloides ; cell-wall ; saccharomyces-cerevisiae ; identification ; purification ; dna ; extraction ; culture ; lipids |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310984900003 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117863]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Polytech Univ, Dalian, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Xinping,Yang, Fan,Zhou, Yongjin,et al. Highly-efficient colony PCR method for red yeasts and its application to identify mutations within two leucine auxotroph mutants[J]. yeast,2012,29(11):467-474. |
| APA | Lin, Xinping.,Yang, Fan.,Zhou, Yongjin.,Zhu, Zhiwei.,Jin, Guojie.,...&Zhao, Zongbao Kent.(2012).Highly-efficient colony PCR method for red yeasts and its application to identify mutations within two leucine auxotroph mutants.yeast,29(11),467-474. |
| MLA | Lin, Xinping,et al."Highly-efficient colony PCR method for red yeasts and its application to identify mutations within two leucine auxotroph mutants".yeast 29.11(2012):467-474. |
入库方式： OAI收割
来源：大连化学物理研究所
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