Mass cytometry-based peripheral blood analysis as a novel tool for early detection of solid tumours: a multicentre study
文献类型:期刊论文
作者 | Zhang, Qi2,3,4,5,6,7; Mao, Ye2,3; Lin, Cheng8; Hu, Manyi2; Wang, Yangyang2; Lou, Yu2,3; Kong, Quanming8; Zhang, Jungang9; Li, Junjian10; Zhang, Yuhua11 |
刊名 | GUT |
出版日期 | 2022-09-16 |
ISSN号 | 0017-5749 |
关键词 | tumour markers hepatocellular carcinoma pancreatic cancer |
DOI | 10.1136/gutjnl-2022-327496 |
通讯作者 | Liang, Tingbo(liangtingbo@zju.edu.cn) |
英文摘要 | Objective Early detection of a tumour remains an unmet medical need, and approaches with high sensitivity and specificity are urgently required. Mass cytometry time-of-flight (CyTOF) is a powerful technique to profile immune cells and could be applied to tumour detection. We attempted to establish diagnostic models for hepatocellular carcinoma (HCC) and pancreatic ductal adenocarcinoma (PDAC). Design We performed CyTOF analysis for 2348 participants from 15 centres, including 1131 participants with hepatic diseases, 584 participants with pancreatic diseases and 633 healthy volunteers. Diagnostic models were constructed through random forest algorithm and validated in subgroups. Results We determined the disturbance of systemic immunity caused by HCC and PDAC, and calculated a peripheral blood immune score (PBIScore) based on the constructed model. The PBIScore exhibited good performance in detecting HCC and PDAC, with both sensitivity and specificity being around 80% in the validation cohorts. We further established an integrated PBIScore (iPBIScore) by combining PBIScore and alpha-fetoprotein or carbohydrate antigen 19-9. The iPBIScore for HCC had an area under the curve (AUC) of 0.99, 0.97 and 0.96 in training, internal validation and external validation cohorts, respectively. Similarly, the iPBIScore for PDAC showed an AUC of 0.99, 0.98 and 0.97 in the training, internal validation and external validation cohorts, respectively. In early-stage and tumour-marker-negative patients, our iPBIScore-based models also showed an AUC of 0.95-0.96 and 0.81-0.92, respectively. Conclusion Our study proved that the alterations of peripheral immune cell subsets could assist tumour detection, and provide a ready-to-use detection model for HCC and PDAC. |
WOS关键词 | ALPHA-FETOPROTEIN LEVELS ; HEPATITIS-C ; T-CELLS ; CANCER ; SERUM ; PROGNOSIS ; DIAGNOSIS ; DIFFERENTIATION ; PHENOTYPE ; CARCINOMA |
资助项目 | National Key Research and Development Program of China[2020YFA0804300] ; National Key Research and Development Program of China[2020YFA0804301] ; National Key Research and Development Program of China[2019YFC1315800] ; National Key Research and Development Program of China[2019YFC1315802] ; National Natural Science Foundation of China[82188102] ; National Natural Science Foundation of China[U20A20378] ; Key Research & Development Program of Zhejiang Province[2021C03063] ; Key Research & Development Program of Zhejiang Province[2019C03019] ; Zhejiang Provincial Natural Science Foundation of China[HDMD22H319373] ; Joint Funds of the Zhejiang Provincial Natural Science Foundation of China[HDMD22H319373] ; Key Research Project of Zhejiang Lab[2022ND0AC01] ; Alibaba Cloud |
WOS研究方向 | Gastroenterology & Hepatology |
语种 | 英语 |
出版者 | BMJ PUBLISHING GROUP |
WOS记录号 | WOS:000855282500001 |
资助机构 | National Key Research and Development Program of China ; National Natural Science Foundation of China ; Key Research & Development Program of Zhejiang Province ; Zhejiang Provincial Natural Science Foundation of China ; Joint Funds of the Zhejiang Provincial Natural Science Foundation of China ; Key Research Project of Zhejiang Lab ; Alibaba Cloud |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/128972] |
专题 | 中国科学院合肥物质科学研究院 |
通讯作者 | Liang, Tingbo |
作者单位 | 1.Hebei Med Univ, Dept Hepatobiliary & Pancreat Surg, Hosp 2, Shijiazhuang, Hebei, Peoples R China 2.Zhejiang Univ, Affiliated Hosp 1, Dept Hepatobiliary & Pancreat Surg, Sch Med, 79 Qingchun Rd, Hangzhou 310003, Zhejiang, Peoples R China 3.Zhejiang Univ, Affiliated Hosp 1, Zhejiang Prov Key Lab Pancreat Dis, Sch Med, Hangzhou, Peoples R China 4.Zhejiang Clin Res Ctr Hepatobiliary & Pancreat Di, Hangzhou, Peoples R China 5.Innovat Ctr Study Pancreat Dis Zhejiang Prov, Hangzhou, Peoples R China 6.Zhejiang Univ, Canc Ctr, Hangzhou, Peoples R China 7.Alibaba Zhejiang Univ Joint Res Ctr Future Digita, Hangzhou, Peoples R China 8.Zhejiang Puluoting Hlth Technol Co Ltd, Hangzhou, Peoples R China 9.Hangzhou Med Coll, Affiliated Peoples Hosp, Zhejiang Prov Peoples Hosp, Dept Hepatobiliary & Pancreat Surg & Minimally In, Hangzhou, Peoples R China 10.Wenzhou Univ, Affiliated Hosp 1, Dept Hepatobiliary & Pancreat Surg, Sch Med, Wenzhou, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Qi,Mao, Ye,Lin, Cheng,et al. Mass cytometry-based peripheral blood analysis as a novel tool for early detection of solid tumours: a multicentre study[J]. GUT,2022. |
APA | Zhang, Qi.,Mao, Ye.,Lin, Cheng.,Hu, Manyi.,Wang, Yangyang.,...&Liang, Tingbo.(2022).Mass cytometry-based peripheral blood analysis as a novel tool for early detection of solid tumours: a multicentre study.GUT. |
MLA | Zhang, Qi,et al."Mass cytometry-based peripheral blood analysis as a novel tool for early detection of solid tumours: a multicentre study".GUT (2022). |
入库方式: OAI收割
来源:合肥物质科学研究院
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