Enhancement of Vitamin K2 Efflux in Bacillus subtilis Natto via a Potential Protein Receptor for Increased Yield
文献类型：期刊论文
| 作者 | Li, Chu1,2; Wang, Han2; Ma, Guoliang2; Wang, Li2; Zheng, Zhiming2 ; Zhao, Genhai2; Wang, Peng2
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| 刊名 | JOURNAL OF FOOD QUALITY
 |
| 出版日期 | 2022-09-16 |
| 卷号 | 2022 |
| ISSN号 | 0146-9428 |
| DOI | 10.1155/2022/8407829 |
| 通讯作者 | Wang, Han(wanghan@ipp.ac.cn) ; Zheng, Zhiming(zhengzhiming2014@163.com) |
| 英文摘要 | Bacillus subtilis is one of the few strains that can secrete synthetic menaquinone-7 (MK-7) to the outside of the cell, and its purpose and mechanism have not been clearly studied. As an amphiphilic protein naturally synthesized by Bacillus subtilis, the BslA protein may be involved in the inversion of extracellular vitamin K2 solubility. The protein structure in UniProt was used to search for the possible binding sites of MK-7, and the analysis of the higher ranking results of the genetic algorithm showed that the ASP166 residue was likely to be the binding site. They could form a stable hydrogen bond connection through ASP166, and approximately 7 proteins formed the conformation of a fixed naphthoquinone ring. We isolated and obtained the BslA protein by Ni-NTA affinity chromatography. Then, MK-7 was modified by BslA in vitro. A series of experiments, such as SEM, XPS, and WCA, showed that MK-7 and BslA proteins can realize self-assembly and transform from fat-soluble to water-soluble complexes. When the bslA protein in Bacillus subtilis natto was overexpressed, its MK-7 synthesis ability was further improved, especially the extracellular MK-7 content, which increased by 16%. This finding suggests that the BslA protein in Bacillus subtilis is likely to be involved in the extracellular secretion of MK-7 as a receptor. |
| WOS关键词 | MENAQUINONE-7 ; CELLS |
| 资助项目 | National Key Research and Development Program of China[2019YFA0904304] ; National Natural Science Foundation of Anhui China[1908085MB43] ; Major Projects of Science and Technology in Anhui Province[202103a06020003] ; National Natural Science Foundation of China[32070088] |
| WOS研究方向 | Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000861569300003 |
| 出版者 | WILEY-HINDAWI |
| 资助机构 | National Key Research and Development Program of China ; National Natural Science Foundation of Anhui China ; Major Projects of Science and Technology in Anhui Province ; National Natural Science Foundation of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/129107]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Wang, Han; Zheng, Zhiming |
| 作者单位 | 1.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chu,Wang, Han,Ma, Guoliang,et al. Enhancement of Vitamin K2 Efflux in Bacillus subtilis Natto via a Potential Protein Receptor for Increased Yield[J]. JOURNAL OF FOOD QUALITY,2022,2022. |
| APA | Li, Chu.,Wang, Han.,Ma, Guoliang.,Wang, Li.,Zheng, Zhiming.,...&Wang, Peng.(2022).Enhancement of Vitamin K2 Efflux in Bacillus subtilis Natto via a Potential Protein Receptor for Increased Yield.JOURNAL OF FOOD QUALITY,2022. |
| MLA | Li, Chu,et al."Enhancement of Vitamin K2 Efflux in Bacillus subtilis Natto via a Potential Protein Receptor for Increased Yield".JOURNAL OF FOOD QUALITY 2022(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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