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A spiropyran with low pK(a) for tracking DNA G-quadruplexes and revealing the dissipation of Delta Psi m with senescence using an in-situ switching strategy

文献类型：期刊论文

作者Tian, Xinrong2; Li, Jin1,2; Zhang, Yanhui2; Gao, Ying2; Afzal, Muhammad Wasim2; Wang, Aoli3; James, Tony D.4; Bai, Yinjuan2; Guo, Yuan2
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2022-05-15
卷号359
关键词Fluorescent probes G-quadruplexes Membrane potential of mitochondria Senescence Senescent cells
DOI10.1016/j.snb.2022.131618
通讯作者Bai, Yinjuan(baiyinjuan@nwu.edu.cn) ; Guo, Yuan(guoyuan@nwu.edu.cn)
英文摘要The in-situ fluorescence triggering of bioprobes only using endogenous bioforces is an ideal non-destructive real-time detection method, which is of particular interest to improve the accuracy of clinical diagnosis and treatment. We have recently reported a strategy of spiropyran in-situ switching triggered by endogenous biological forces in vivo to develop optical probes for this purpose. However, such probes, as with all spiropyrans, are sensitive to lysosomal acidity. We here present a spiropyran-based fluorescent probe TANG with low pK(a), which can recognize intranuclear DNA G4s in situ without the aid of exogenous light or chemicals and is as stable to lysosomal acidity due to a decreased pK(a) value (4.3). Interestingly, despite the stability to lysosomal pH environment, the TANG spiropyran can be opened in situ by the negative membrane potential of extranuclear mitochondria (Delta Psi m), causing a ratiometric change in fluorescence signals and providing the in-situ and real-time tracking of Delta Psi m. Of note, ratiometric imaging using TANG indicates that Delta Psi m decreases gradually with cellular senescence, which is to the best of our knowledge the first visualization of such mitochondrion-related aging processes using a ratiometric imaging approach.
WOS关键词FLUORESCENT-PROBE ; MITOCHONDRIAL ; PH ; SENSOR ; CELLS
资助项目NSF of China[21977082] ; NSF of China[22037002] ; Natural Science Basic Research Plan in Shaanxi Province of China[2020JC-38] ; Technology Plan Project of Xi'an of China[2019218214GXRC018CG019-GXYD18.1] ; Royal Society
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:000860649000007
出版者ELSEVIER SCIENCE SA
资助机构NSF of China ; Natural Science Basic Research Plan in Shaanxi Province of China ; Technology Plan Project of Xi'an of China ; Royal Society
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/129177]  
专题中国科学院合肥物质科学研究院
通讯作者Bai, Yinjuan; Guo, Yuan
作者单位1.Xian Univ Sci & Technol, Coll Chem & Chem Engn, Xian 710054, Peoples R China
2.Northwest Univ, Coll Chem & Mat Sci, Natl Demonstrat Ctr Expt Chem Educ, Key Lab Synthet & Nat Funct Mol,Minist Educ, Xian 710127, Peoples R China
3.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Peoples R China
4.Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England
推荐引用方式
GB/T 7714		 Tian, Xinrong,Li, Jin,Zhang, Yanhui,et al. A spiropyran with low pK(a) for tracking DNA G-quadruplexes and revealing the dissipation of Delta Psi m with senescence using an in-situ switching strategy[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,359.
APA Tian, Xinrong.,Li, Jin.,Zhang, Yanhui.,Gao, Ying.,Afzal, Muhammad Wasim.,...&Guo, Yuan.(2022).A spiropyran with low pK(a) for tracking DNA G-quadruplexes and revealing the dissipation of Delta Psi m with senescence using an in-situ switching strategy.SENSORS AND ACTUATORS B-CHEMICAL,359.
MLA Tian, Xinrong,et al."A spiropyran with low pK(a) for tracking DNA G-quadruplexes and revealing the dissipation of Delta Psi m with senescence using an in-situ switching strategy".SENSORS AND ACTUATORS B-CHEMICAL 359(2022).

入库方式： OAI收割

来源：合肥物质科学研究院

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