Cuprous ions can disrupt the structure and functions of the RING finger domain of RNF11
文献类型：期刊论文
| 作者 | Wang, Yu2 ; Hu, Hongze2; Yuan, Siming3; Li, Yunyan3; Cao, Kaiming2; Sun, Hongbin1; Liu, Yangzhong2
|
| 刊名 | INORGANIC CHEMISTRY FRONTIERS
 |
| 出版日期 | 2022-06-06 |
| ISSN号 | 2052-1553 |
| DOI | 10.1039/d2qi00631f |
| 通讯作者 | Liu, Yangzhong(liuyz@ustc.edu.cn) |
| 英文摘要 | Copper is an essential element that plays crucial roles in a variety of biological processes, while excessive copper is harmful to cells. RNF11 is a RING finger protein associated with cell proliferation and development and metastasis of cancer cells. Here we find that cuprous ions can bind to the RING finger domain of RNF11 with a high binding affinity (disassociation constant 1.80 x 10(-17) M). Cuprous ions are capable of depleting zinc ions from RNF11 and disrupting the structure and function of the protein. Circular dichroism and NMR measurements indicate that Cu(i) binding disrupts the secondary structures of RNF11 and further induces protein aggregation. NMR titration confirms that Cu(i) binds to the Zn(ii) coordination residues. Consequently, cuprous binding interferes with the formation of RNF11/UBE2N complexes, a crucial step of the ubiquitination process. In addition, cellular assays confirmed that copper ions affect its function as a transcriptional regulator for SEC23B, SEC24B, and SEC24D. This result indicates that the alteration of copper homeostasis in cells could influence the RNF11-associated regulation processes. |
| WOS关键词 | PROTEIN RNF11 ; CELL-DEATH ; XIAP |
| 资助项目 | National Key R&D Program of China[2017YFA0505400] ; National Key R&D Program of China[2020YFA0710700] ; National Science Foundation of China[22177109] ; National Science Foundation of China[21877103] ; National Science Foundation of China[52021002] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000813557100001 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | National Key R&D Program of China ; National Science Foundation of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/129568]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Liu, Yangzhong |
| 作者单位 | 1.Zhengzhou Univ Light Ind, Sch Food & Biol Engn, Zhengzhou, Peoples R China 2.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yu,Hu, Hongze,Yuan, Siming,et al. Cuprous ions can disrupt the structure and functions of the RING finger domain of RNF11[J]. INORGANIC CHEMISTRY FRONTIERS,2022. |
| APA | Wang, Yu.,Hu, Hongze.,Yuan, Siming.,Li, Yunyan.,Cao, Kaiming.,...&Liu, Yangzhong.(2022).Cuprous ions can disrupt the structure and functions of the RING finger domain of RNF11.INORGANIC CHEMISTRY FRONTIERS. |
| MLA | Wang, Yu,et al."Cuprous ions can disrupt the structure and functions of the RING finger domain of RNF11".INORGANIC CHEMISTRY FRONTIERS (2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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