Whole-Genome Sequencing Reveals Germ Cell Mutagenicity of alpha-Endosulfan in Caenorhabditis elegans
文献类型:期刊论文
作者 | Cao, Zhenxiao2,3,4; Wang, Meimei1; Zhou, Tong3,4; Xu, An3,4; Du, Hua4 |
刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY |
出版日期 | 2022-10-14 |
ISSN号 | 0013-936X |
关键词 | alpha-endosulfan Caenorhabditis elegans germ cell mutagenicity next-generation sequencing |
DOI | 10.1021/acs.est.2c06817 |
通讯作者 | Xu, An(anxu@ipp.ac.cn) ; Du, Hua(huadu@ipp.ac.cn) |
英文摘要 | Endosulfan is an extensively used organochlorine pesticide around the world, which was classified as a persistent organic pollutant (POP) in 2009. Although previous studies have documented the reproductive toxicity of endosulfan in a variety of organisms, little is known about the influence of endosulfan on the genome stability of germ cells and nonexposed progeny. Here we applied whole-genome sequencing to explore the germ cell mutagenicity of alpha-endosulfan in Caenorhabditis elegans (C. elegans). We found that, although low doses of alpha-endosulfan exhibited a minor effect on the reproductive capacity of C. elegans, chronic exposure to 1 mu M alpha-endosulfan significantly increased the mutation frequencies of nonexposed progeny. Further analysis of genome-wide mutation spectra demonstrated that alpha- endosulfan preferentially elicited A:T -> G:C substitutions and clustered mutations. By using worms deficient in DNA damage response genes, our results suggest the involvement of translesion synthesis polymerase ri in modulating alpha-endosulfan-induced mutations in germ cells. Together, these observations reveal the germ cell mutagenicity of alpha-endosulfan in C. elegans and the possible underlying mechanism. In addition, our findings implicate that germ cell mutagenicity might be a necessary consideration for the health risk assessment of environmental chemicals such as POPs. |
WOS关键词 | ORGANOCHLORINE PESTICIDES ; REPRODUCTIVE TOXICITY ; DNA-DAMAGE ; TRANSLESION ; GENOTOXICITY ; DEGRADATION ; INSECTICIDE ; ISOMERS ; ETA ; AIR |
资助项目 | National Natural Science Foundation of China[22006149] ; Hundred Talents Program[E09BR22] ; Anhui Provincial Natural Science Foundation[2008085MB49] ; Anhui Medical University Research Enhancement Program[2021xkjT004] |
WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000875545100001 |
资助机构 | National Natural Science Foundation of China ; Hundred Talents Program ; Anhui Provincial Natural Science Foundation ; Anhui Medical University Research Enhancement Program |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/129787] |
专题 | 中国科学院合肥物质科学研究院 |
通讯作者 | Xu, An; Du, Hua |
作者单位 | 1.Anhui Med Univ, Sch Basic Med Sci, Dept Pathophysiol, Hefei 230032, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Environm Sci & Optoelect Technol, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Contr, Hefei 230031, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | Cao, Zhenxiao,Wang, Meimei,Zhou, Tong,et al. Whole-Genome Sequencing Reveals Germ Cell Mutagenicity of alpha-Endosulfan in Caenorhabditis elegans[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2022. |
APA | Cao, Zhenxiao,Wang, Meimei,Zhou, Tong,Xu, An,&Du, Hua.(2022).Whole-Genome Sequencing Reveals Germ Cell Mutagenicity of alpha-Endosulfan in Caenorhabditis elegans.ENVIRONMENTAL SCIENCE & TECHNOLOGY. |
MLA | Cao, Zhenxiao,et al."Whole-Genome Sequencing Reveals Germ Cell Mutagenicity of alpha-Endosulfan in Caenorhabditis elegans".ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022). |
入库方式: OAI收割
来源:合肥物质科学研究院
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