Single Chain Variable Fragment Displaying M13 Phage Library Functionalized Magnetic Microsphere-Based Protein Equalizer for Human Serum Protein Analysis
文献类型：期刊论文
| 作者 | Zhu, Guijie1; Zhao, Peng1; Deng, Nan1,2; Tao, Dingyin1; Sun, Liangliang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2012-09-18 |
| 卷号 | 84期号:18页码:7633-7637 |
| 产权排序 | 1,1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | single chain variable fragment (scfv) displaying the m13 phage library was covalently immobilized on magnetic microspheres and used as a protein equalizer for the treatment of human serum. first, scfv displaying m13 phage library functionalized magnetic microspheres (scfv@m13@mm) was incubated with a human serum sample. second, captured proteins on scfv@m13@mm were eluted with 2 m nacl, so mm glycine-hydrochloric acid (gly-hcl), and 20% (v/v) acetonitrile with 0.5% (v/v) trifluoroacetic acid in sequence. finally, the tightly bonded proteins were released by the treatment with thrombin. the eluates were first analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) with silver staining. results indicated that the difference of protein concentration was reduced obviously in nacl and gly-hcl fractions compared with untreated human serum sample. the eluates were also digested with trypsin, followed by online 2d-strong cation exchange (scx)-rplc-esi-ms/ms analysis. results demonstrated that the number of proteins identified from an scfv@m13@mm treated human serum sample was improved 100% compared with that from the untreated sample. in addition, the spectral count of 10 high abundance proteins (serum albumin, serotransferrin, alpha-2-macroglobulin, alpha-1-antitrypsin, apolipoprotein b-100, ig gamma-2 chain c region, haptoglobin, hemopodn, alpha-1-acid glycoprotein 1, and alpha-2-hs-glycoprotein) decreased evidently after scfv@m13@mm treatment. all these results demonstrate that scfv@m13@mm could efficiently remove high-abundance proteins, reduce the protein concentration difference of human serum, and result in more protein identification. |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | peptide ligand libraries ; low-abundance proteome ; in-depth exploration ; chromatography m-lac ; red-blood-cells ; liquid-chromatography ; plasma-proteins ; tof-ms ; depletion ; enrichment |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000308829700009 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117968]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Guijie,Zhao, Peng,Deng, Nan,et al. Single Chain Variable Fragment Displaying M13 Phage Library Functionalized Magnetic Microsphere-Based Protein Equalizer for Human Serum Protein Analysis[J]. analytical chemistry,2012,84(18):7633-7637. |
| APA | Zhu, Guijie.,Zhao, Peng.,Deng, Nan.,Tao, Dingyin.,Sun, Liangliang.,...&Zhang, Yukui.(2012).Single Chain Variable Fragment Displaying M13 Phage Library Functionalized Magnetic Microsphere-Based Protein Equalizer for Human Serum Protein Analysis.analytical chemistry,84(18),7633-7637. |
| MLA | Zhu, Guijie,et al."Single Chain Variable Fragment Displaying M13 Phage Library Functionalized Magnetic Microsphere-Based Protein Equalizer for Human Serum Protein Analysis".analytical chemistry 84.18(2012):7633-7637. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。