IC50: an unsuitable measure for large-sized prostate cancer spheroids in drug sensitivity evaluation
文献类型：期刊论文
| 作者 | Xu, Yipeng2,9,10; Pachnikova, Gabriela1,6,7,8; Wang, He; Wu, Yaoyao5; Przybilla, Dorothea1,6,7,8; Schaefer, Reinhold1,6,7,8; Chen, Zihao4; Zhu, Shaoxing2,9,10; Keilholz, Ulrich1,3,6,7,8 |
| 刊名 | BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES
 |
| 出版日期 | 2022 |
| 卷号 | 22 |
| ISSN号 | 1512-8601 |
| 关键词 | IC50 docetaxel spheroids drug testing preclinical models prostate cancer 2D clinical models 3D clinical models cell culture |
| DOI | 10.17305/bjbms.2022.7279 |
| 通讯作者 | Zhu, Shaoxing(zhusx@zjcc.org.cn) ; Keilholz, Ulrich(ulrich.keilholz@charite.de) |
| 英文摘要 | Preclinical models of tumors have the potential to become valuable tools for commercial drug research and development, and 3D culture systems are gaining traction in this area, particularly in prostate cancer (PCa) research. However, nearly all 3D drug design and screening assessments are based on 2D experiments, suggesting limitations of 3D drug testing. To simulate the natural response of human cells to the drug, we detected the half-maximal inhibitory concentration (IC50) changes of 2D/3D LNCaP cells in the drug docetaxel, as well as the sensitivity of different morphologies of 2D/3D LNCaP to docetaxel treatment. In contrast to 2D LNCaP cells, the evaluation of LNCaP spheroids' susceptibility to treatment was more complicated; the fitness of IC50, curves of 2D and 3D tumor cell preclinical models differs significantly. IC50 curves were unsuitable for large-sized LNCaP spheroids. More evaluation indexes (such as max inhibition) and experiments (such as spheroids formation) should be explored and performed to evaluate the susceptibility systematically. |
| WOS关键词 | PATIENT-DERIVED XENOGRAFTS ; MODELS ; CULTURES ; GROWTH |
| 资助项目 | Natural Science Foundation of Zhejiang Province[LQ20H160007] ; ZAST International Medical Cooperation Programme |
| WOS研究方向 | Research & Experimental Medicine |
| 语种 | 英语 |
| 出版者 | ASSOC BASIC MEDICAL SCI FEDERATION BOSNIA & HERZEGOVINA SARAJEVO |
| WOS记录号 | WOS:000876747500002 |
| 资助机构 | Natural Science Foundation of Zhejiang Province ; ZAST International Medical Cooperation Programme |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/130132]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zhu, Shaoxing; Keilholz, Ulrich |
| 作者单位 | 1.Free Univ Berlin, Berlin, Germany 2.Key Lab Zhejiang Prov Aptamers & Theranost, Hangzhou, Peoples R China 3.German Canc Consortium DKTK, Heidelberg, Germany 4.Southern Med Univ, Dept Urol, Guangzhou, Peoples R China 5.Zhejiang Chinese Med Univ, Clin 2, Med Coll, Hangzhou, Zhejiang, Peoples R China 6.Berlin Inst Hlth, Berlin, Germany 7.Humboldt Univ, Berlin, Germany 8.Charite, Ctr Comprehens Canc, Berlin, Germany 9.Univ Chinese Acad Sci, Canc Hosp, Dept Urol, Zhejiang Canc Hosp, Hangzhou, Peoples R China 10.Chinese Acad Sci, Inst Basic Med & Canc, Hangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Yipeng,Pachnikova, Gabriela,Wang, He,et al. IC50: an unsuitable measure for large-sized prostate cancer spheroids in drug sensitivity evaluation[J]. BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES,2022,22. |
| APA | Xu, Yipeng.,Pachnikova, Gabriela.,Wang, He.,Wu, Yaoyao.,Przybilla, Dorothea.,...&Keilholz, Ulrich.(2022).IC50: an unsuitable measure for large-sized prostate cancer spheroids in drug sensitivity evaluation.BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES,22. |
| MLA | Xu, Yipeng,et al."IC50: an unsuitable measure for large-sized prostate cancer spheroids in drug sensitivity evaluation".BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES 22(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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