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Simplified Cell Magnetic Isolation Assisted SC2 Chip to Realize "Sample in and Chemotaxis Out": Validated by Healthy and T2DM Patients' Neutrophils

文献类型：期刊论文

作者Yang, Xiao1,2; Gao, Chaoru1,2; Liu, Yong1; Zhu, Ling1; Yang, Ke1
刊名MICROMACHINES
出版日期2022-11-01
卷号13
关键词neutrophil microfluidic chip isolation chemotaxis test diabetes mellitus type 2 (T2DM)
DOI10.3390/mi13111820
通讯作者Zhu, Ling(zhul@aiofm.ac.cn) ; Yang, Ke(keyang@aiofm.ac.cn)
英文摘要Neutrophil migration in tissues critically regulates the human immune response and can either play a protective role in host defense or cause health problems. Microfluidic chips are increasingly applied to study neutrophil migration, attributing to their advantages of low reagent consumption, stable chemical gradients, visualized cell chemotaxis monitoring, and quantification. Most chemotaxis chips suffered from low throughput and fussy cell separation operations. We here reported a novel and simple "sample in and chemotaxis out" method for rapid neutrophils isolation from a small amount of whole blood based on a simplified magnetic method, followed by a chemotaxis assay on a microfluidic chip (SC2 chip) consisting of six cell migration units and six-cell arrangement areas. The advantages of the "sample in and chemotaxis out" method included: less reagent consumption (10 mu L of blood + 1 mu L of magnetic beads + 1 mu L of lysis buffer); less time (5 min of cell isolation + 15 min of chemotaxis testing); no ultracentrifugation; more convenient; higher efficiency; high throughput. We have successfully validated the approach by measuring neutrophil chemotaxis to frequently-used chemoattractant (i.e., fMLP). The effects of D-glucose and mannitol on neutrophil chemotaxis were also analyzed. In addition, we demonstrated the effectiveness of this approach for testing clinical samples from diabetes mellitus type 2 (T2DM) patients. We found neutrophils' migration speed was higher in the "well-control" T2DM than in the "poor-control" group. Pearson coefficient analysis further showed that the migration speed of T2DM was negatively correlated with physiological indicators, such as HbA1c (-0.44), triglyceride (-0.36), C-reactive protein (-0.28), and total cholesterol (-0.28). We are very confident that the developed "sample in and chemotaxis out" method was hoped to be an attractive model for analyzing the chemotaxis of healthy and disease-associated neutrophils.
WOS关键词MIGRATION ASSAY ; DROP ; BLOOD ; KIT
资助项目National Natural Science Foundation of China[31800713] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2021442]
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation ; Physics
语种英语
出版者MDPI
WOS记录号WOS:000881273400001
资助机构National Natural Science Foundation of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/130188]  
专题中国科学院合肥物质科学研究院
通讯作者Zhu, Ling; Yang, Ke
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Inst Opt & Fine Mech, Hefei 230031, Peoples R China
2.Anhui Med Univ, Sch Biomed Engn, Hefei 230032, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Xiao,Gao, Chaoru,Liu, Yong,et al. Simplified Cell Magnetic Isolation Assisted SC2 Chip to Realize "Sample in and Chemotaxis Out": Validated by Healthy and T2DM Patients' Neutrophils[J]. MICROMACHINES,2022,13.
APA Yang, Xiao,Gao, Chaoru,Liu, Yong,Zhu, Ling,&Yang, Ke.(2022).Simplified Cell Magnetic Isolation Assisted SC2 Chip to Realize "Sample in and Chemotaxis Out": Validated by Healthy and T2DM Patients' Neutrophils.MICROMACHINES,13.
MLA Yang, Xiao,et al."Simplified Cell Magnetic Isolation Assisted SC2 Chip to Realize "Sample in and Chemotaxis Out": Validated by Healthy and T2DM Patients' Neutrophils".MICROMACHINES 13(2022).

入库方式： OAI收割

来源：合肥物质科学研究院

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