Duplex real-time fluorescent recombinase polymerase amplification for the rapid and sensitive detection of two resistance genes in drug-resistant Staphylococcus aureus
文献类型：期刊论文
| 作者 | Shi, Zhonglin3,4; Li, Yanan2,4 ; Hu, Anzhong4; Cui, Junsheng4; Shao, Min1; Zhu, Ling4; Yang, Ke4; Liu, Yong4; Deng, Guoqing4; Zhu, Cancan4
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| 刊名 | JOURNAL OF MICROBIOLOGICAL METHODS
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| 出版日期 | 2022-11-01 |
| 卷号 | 202 |
| 关键词 | Drug -resistant Staphylococcus aureus ermA mecA Recombinase polymerase amplification Resistance genes |
| ISSN号 | 0167-7012 |
| DOI | 10.1016/j.mimet.2022.106590 |
| 通讯作者 | Zhu, Cancan(zhucancan@aiofm.ac.cn) |
| 英文摘要 | In the clinic, drug-resistant Staphylococcus aureus (S. aureus) is the most common suppurative infection pathogen in humans. It can cause local infections in humans and animals, such as pneumonia, mastitis, and other systemic illnesses. At present, the detection of drug-resistant S. aureus includes traditional isolation by culture and anti-microbial susceptibility tests. However, these methods are complicated in experimental design, specialized in operation and time consuming. Therefore, a rapid and accurate drug-resistant S. aureus detection technology is urgently needed. In this study, we combined duplex pairs of fluorescent probes with recombinase polymerase amplification (RPA) to realize the simultaneous detection of two resistance genes in drug-resistant S. aureus. The method shows low detection limit, detecting 20 copies within 10 min. The analytical specificity of this method was evaluated with several related drug-resistant bacterial strains (Non-resistant S. aureus, Acinetobacter bau-mannii, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae), and the positive signal was only observed with drug-resistant S. aureus. In addition, the clinical suitability of this method was verified by 30 clinical isolates. Compared with qPCR, the coincidence rate of drug resistance genes were 100% (mecA) and 96.7% (ermA), respectively. These results show that the duplex real-time fluorescent RPA assay is a rapid, low detection limit and specific detection of mecA and ermA genes in isolates of drug-resistant S. aureus. |
| WOS关键词 | ISOTHERMAL AMPLIFICATION ; CHAIN-REACTION ; ASSAY ; PATHOGENS ; POINT ; DNA |
| 资助项目 | Natural Science Foundation of Anhui Province of China[82002189] ; Key Research and Development Program of Anhui Province of China[2008085QF311] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[202004d7020014] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2018489] ; Dean's Fund of Hefei Institute of Physical Science, China[2021442] ; National Natural Science Foundation of China[YZJJ2020QN35] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000877555600008 |
| 出版者 | ELSEVIER |
| 资助机构 | Natural Science Foundation of Anhui Province of China ; Key Research and Development Program of Anhui Province of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Dean's Fund of Hefei Institute of Physical Science, China ; National Natural Science Foundation of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/130307]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zhu, Cancan |
| 作者单位 | 1.Anhui Med Univ, Affiliated Hosp 1, Dept Crit Care Med, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 3.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Anhui, Peoples R China 4.Chinese Acad Sci, Anhui Inst Opt & Fine Mech, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Zhonglin,Li, Yanan,Hu, Anzhong,et al. Duplex real-time fluorescent recombinase polymerase amplification for the rapid and sensitive detection of two resistance genes in drug-resistant Staphylococcus aureus[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2022,202. |
| APA | Shi, Zhonglin.,Li, Yanan.,Hu, Anzhong.,Cui, Junsheng.,Shao, Min.,...&Zhu, Cancan.(2022).Duplex real-time fluorescent recombinase polymerase amplification for the rapid and sensitive detection of two resistance genes in drug-resistant Staphylococcus aureus.JOURNAL OF MICROBIOLOGICAL METHODS,202. |
| MLA | Shi, Zhonglin,et al."Duplex real-time fluorescent recombinase polymerase amplification for the rapid and sensitive detection of two resistance genes in drug-resistant Staphylococcus aureus".JOURNAL OF MICROBIOLOGICAL METHODS 202(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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