Hydrophilic modification of silica-titania mesoporous materials as restricted-access matrix adsorbents for enrichment of phosphopeptides
文献类型：期刊论文
| 作者 | Wang, Fei1; Guan, Yafeng2; Zhang, Sen3; Xia, Yan1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2012-07-13 |
| 卷号 | 1246页码:76-83 |
| 关键词 | Restricted-access matrix (RAM) adsorbents Mesoporous materials (Ti-MCM-41) Hydrophilic Modification Phosphopeptide enrichment Tryptic digestion |
| 产权排序 | 2,2 |
| 通讯作者 | yanxia |
| 英文摘要 | a new nano-scale restricted-access matrix (ram) sio2 (mcm-41) with relatively high ti-content (ti/si = 0.1), but superior surface area (1129 m(2)/g), was successfully synthesised for the enrichment of phosphopeptides. the tio2 was incorporated into the si-mcm-41 via a hydrothermal process and the external surface was modified with alkyl diol by the successive hydrolysis of gamma-(glycidyloxypropyl) trimethoxysilane (gptms). scanning electron microscopy, transmission electron microscopy, n-2 adsorption and fourier transform infrared spectroscopy were used to characterise the alkyl diol-ti-mcm-41. the appropriate pore diameter (2 nm) coupled to the marshy weeds-like hydrophilic external surface result in an efficient size-exclusion effect for the adsorption of standard cytochrome c with a molecular weight (mw) of ca. 12.4 kda. at the same time, the strong affinity interaction between the incorporated titanium in the framework and the phosphoryl groups of phosphopeptides demonstrated a selective extract of phosphopeptides from the tryptic digestion. the detection sensitivity for phosphopeptides, determined by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof ms) was as low as 5 fmol for standard tryptic digest of beta-casein. therefore, this alkyl diol-ti-mcm-41 mesoporous material can be used as a potential adsorbent for applications in ms-based phosphoproteomics. (c) 2012 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | ion affinity-chromatography ; protein-phosphorylation ; liquid-chromatography ; surface-area ; catalysts ; column ; epoxidation ; functionalization ; phosphoproteome ; phosphates |
| 收录类别 | SCI ; ISTP |
| 语种 | 英语 |
| WOS记录号 | WOS:000306246000013 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117979]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Res Ctr Analyt Sci, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Nankai Univ, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fei,Guan, Yafeng,Zhang, Sen,et al. Hydrophilic modification of silica-titania mesoporous materials as restricted-access matrix adsorbents for enrichment of phosphopeptides[J]. journal of chromatography a,2012,1246:76-83. |
| APA | Wang, Fei,Guan, Yafeng,Zhang, Sen,&Xia, Yan.(2012).Hydrophilic modification of silica-titania mesoporous materials as restricted-access matrix adsorbents for enrichment of phosphopeptides.journal of chromatography a,1246,76-83. |
| MLA | Wang, Fei,et al."Hydrophilic modification of silica-titania mesoporous materials as restricted-access matrix adsorbents for enrichment of phosphopeptides".journal of chromatography a 1246(2012):76-83. |
入库方式： OAI收割
来源：大连化学物理研究所
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