Article Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus
文献类型：期刊论文
| 作者 | Gao, Chao2; Gu, Jinge3; Zhang, Hong2; Jiang, Kai2; Tang, Linlin2; Liu, Ren4; Zhang, Li4; Zhang, Pengfei4; Liu, Cong1,3,5; Dai, Bin2 |
| 刊名 | CELL REPORTS
 |
| 出版日期 | 2022-07-19 |
| 卷号 | 40 |
| ISSN号 | 2211-1247 |
| DOI | 10.1016/j.celrep.2022.111086 |
| 通讯作者 | Liu, Cong(liulab@sioc.ac.cn) ; Dai, Bin(daibin@sjtu.edu.cn) ; Song, Jie(sjie@sjtu.edu.cn) |
| 英文摘要 | Hyperosmotic stress as physiologic dysfunction can reduce the cell volume and then redistribute both protein concentration and ionic strength, but its effect on liquid-liquid phase separation (LLPS) is not well un-derstood. Here, we map the hyperosmotic-stress-induced nuclear LLPS of amyotrophic lateral sclerosis (ALS)-related proteins (fused in sarcoma [FUS], TAR DNA-binding protein 43 [TDP-43]). The dynamic and reversibility of FUS granules are continuable with the increase of hypertonic stimulation time, but those of TDP-43 granules decrease significantly. Strikingly, FUS granules, but not TDP-43 granules, contain essential chaperone Hsp40, which can protect amyloid protein from solid aggregation. Moreover, FUS nuclear gran-ules can co-localize with paraspeckles, but not promyelocytic leukemia (PML) bodies or nuclear speckles, while TDP-43 nuclear granules cannot co-localize with the above nuclear bodies. Together, these results may broaden our understanding of the LLPS of ALS-related proteins in response to cellular stress. |
| WOS关键词 | DNA-BINDING PROTEIN ; GRANULES ; TDP-43 ; GENE ; FUS ; TRANSITION ; TELOMERES ; CHAPERONE ; PROMOTES ; DROPLETS |
| 资助项目 | National Natural Science Foundation of China[82188101] ; National Natural Science Foundation of China[22161132008] ; National Key R&D Program of China[2021YFF1200200] ; Natural Science Foundation of Shanghai, China[19520714100] ; Natural Science Foundation of Shanghai, China[19ZR1475800] ; Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study[SNZJU-SIAS-006] ; Science and Technology Commission of Shanghai Municipality (STCSM)[20XD1425000] ; Science and Technology Commission of Shanghai Municipality (STCSM)[2019SHZDZX02] ; CAS project for Young Scientists in Basic Research[YSBR-009] ; Shanghai Pilot Program for Basic Research - Chinese Anatomical Society, Shanghai Branch[CYJ-SHFY-2022-005] ; Shanghai Pujiang Program[19PJ1405000] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| 出版者 | CELL PRESS |
| WOS记录号 | WOS:000883795600002 |
| 资助机构 | National Natural Science Foundation of China ; National Key R&D Program of China ; Natural Science Foundation of Shanghai, China ; Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study ; Science and Technology Commission of Shanghai Municipality (STCSM) ; CAS project for Young Scientists in Basic Research ; Shanghai Pilot Program for Basic Research - Chinese Anatomical Society, Shanghai Branch ; Shanghai Pujiang Program |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/130421]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Liu, Cong; Dai, Bin; Song, Jie |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Dept Neurol, Shanghai 200025, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Inst Nano Biomed & Engn, Dept Instrument Sci & Engn, Shanghai 200240, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Organ Chem, Interdisciplinary Res Ctr Biol & Chem, Shanghai 201210, Peoples R China 4.Chinese Acad Sci, Univ Chinese Acad Sci, Canc Hosp, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China 5.Shanghai Jiao Tong Univ, Ruijin Hosp, Inst Neurol, Sch Med, Shanghai 200025, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Chao,Gu, Jinge,Zhang, Hong,et al. Article Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus[J]. CELL REPORTS,2022,40. |
| APA | Gao, Chao.,Gu, Jinge.,Zhang, Hong.,Jiang, Kai.,Tang, Linlin.,...&Song, Jie.(2022).Article Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus.CELL REPORTS,40. |
| MLA | Gao, Chao,et al."Article Hyperosmotic-stress-induced liquid-liquid phase separation of ALS-related proteins in the nucleus".CELL REPORTS 40(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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