Picomolar thrombin detection by orchestration of triple signal amplification strategy with hierarchically porous Ti3C2Tx MXene electrode material-catalytic hairpin assembly reaction-metallic nanoprobes
文献类型：期刊论文
| 作者 | Yang, Pengqi2,3; Guo, Xinyue2,3; Zhang, Jia1,2; Chen, Chaowen1,2; Gan, Yuehao2,3; Xie, Wenteng2,3; Du, Yue1,2; Wu, Zhengyan1,2
|
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2022-07-15 |
| 卷号 | 208 |
| ISSN号 | 0956-5663 |
| 关键词 | Hierarchically porous MXene framework Metallic nanoprobes Synergistic amplification strategy Electrochemical aptasensor Thrombin detection |
| DOI | 10.1016/j.bios.2022.114228 |
| 通讯作者 | Zhang, Jia(zhangj@iim.ac.cn) ; Wu, Zhengyan(zywu@ipp.ac.cn) |
| 英文摘要 | Signal amplification strategies are essential to boost the sensitivity of detecting targeted ions or molecules with important biological functions, while few studies take advantage of signal amplification strategies more than two. As a "proof-of-concept" demonstration, we present the ultrasensitive electrochemical aptasensor for picomolar thrombin detection by synchronous coordination of triple signal amplification strategy. The porous MXene framework (PMXF) with secondary pores is constructed as carrier to increase electrons transfer channels, and thionine (as redox indicator) labelled Au nanorod (AuNR) and hollow Cu-Pt alloy (HCuPtA) are synthesized as the electrical signal amplifiers to enhance the response signals. In the presence of picomolar-level thrombin, catalytic hairpin assembly reactions of DNA are triggered to bridge thionine labelled AuNR or HCuPtA nanoprobes on the PMXF with controllablly scondary pore structures. Under the optimal conditionals, the sandwichtyped aptasensor based on PMXF-5/AuNR shows a more low limit of detection (LOD) of 0.67 pM with a linear range from 2 pM to 10 nM, while PMXF-5/HcuPtA exhibits a more wide linear range from 50 pM to 50 nM with a LOD of 16.67 pM for thormbin. This sensing platform can be customized to analyze other biological or environmental substances at an ultrahigh level by rationally designing DNA sequences of target-binding aptamer. |
| WOS关键词 | HIGH-PERFORMANCE ; LABEL-FREE ; APTASENSOR ; FABRICATION ; TI2CTX |
| 资助项目 | University Synergy Innovation Program of Anhui Province[GXXT-2021-059] ; Special Project of Central Government for Local Science and Technology Development of Shandong Province[YDZX20203700001291] ; Open Research Fund of Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| WOS记录号 | WOS:000792492900004 |
| 资助机构 | University Synergy Innovation Program of Anhui Province ; Special Project of Central Government for Local Science and Technology Development of Shandong Province ; Open Research Fund of Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/130783]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zhang, Jia; Wu, Zhengyan |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230031, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 3.Univ Sci & Technol China, Hefei 230026, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Pengqi,Guo, Xinyue,Zhang, Jia,et al. Picomolar thrombin detection by orchestration of triple signal amplification strategy with hierarchically porous Ti3C2Tx MXene electrode material-catalytic hairpin assembly reaction-metallic nanoprobes[J]. BIOSENSORS & BIOELECTRONICS,2022,208. |
| APA | Yang, Pengqi.,Guo, Xinyue.,Zhang, Jia.,Chen, Chaowen.,Gan, Yuehao.,...&Wu, Zhengyan.(2022).Picomolar thrombin detection by orchestration of triple signal amplification strategy with hierarchically porous Ti3C2Tx MXene electrode material-catalytic hairpin assembly reaction-metallic nanoprobes.BIOSENSORS & BIOELECTRONICS,208. |
| MLA | Yang, Pengqi,et al."Picomolar thrombin detection by orchestration of triple signal amplification strategy with hierarchically porous Ti3C2Tx MXene electrode material-catalytic hairpin assembly reaction-metallic nanoprobes".BIOSENSORS & BIOELECTRONICS 208(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。