Stepwise tuning of a molecular beacon coupled Y probe regulates ternary DNA nanomachine-based microRNA determination
文献类型：期刊论文
| 作者 | Wang, Qi2,4,5; Li, Wenyong5; Xia, Juan5; Wu, Hai5; Wu, Lijun2,3,4 ; Xu, Jianguo1
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| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2022-07-15 |
| 卷号 | 363 |
| 关键词 | Y shape probe Ternary DNA nanomachine MiRNA determination Isothermal all -in amplification Fluorescent sensing |
| DOI | 10.1016/j.snb.2022.131858 |
| 通讯作者 | Wu, Lijun(ljw@ipp.ac.cn) ; Xu, Jianguo(jgxu0816@163.com) |
| 英文摘要 | The construction of DNA nanodevices is vital for disease diagnosis and biomedical study. Herein, we proposed a ternary DNA nanomachine for microRNA determination using a molecular beacon (MB) coupled Y shape probe (MB-YP). The MB-YP was a tripartite junction scaffold connected with MB-Y-I, Y-II, and Y-III. The target miRNA firstly recognizes MB-Y-I, activating the MB-Y-I-DNA nanomachine to produce endogenous primer I (EPI) responsible for the activation of Y-II-DNA nanomachine. The Y-II-DNA nanomachine then allows the production of numerous EPII, which in turn activates the Y-III-DNA nanomachine to produce EPIII for re-activating MB-Y-I-DNA nanomachine. The stepwise operated MB-Y-I-DNA nanomachine, Y-II-DNA nanomachine, and Y-III-DNA nanoma-chine constituted the ternary DNA nanomachine and the repeated use of EPI, EPII, and EPIII permits it an all-in amplification. These features lead to the opening of MB-YI for emitting strong fluorescence. As low as 1 fM miRNA can thus be quantitatively detected and the linear response of the miRNA is from 1 fM to 100 pM (six orders of magnitude). Moreover, the nanomachine is specific to distinguish target miRNA from one-base mutant, inserted, and deleted target analogs. Its practicability was demonstrated by confirming miRNA-21 is overex-pressed in clinical human serums from lung cancer patients than healthy donors. |
| WOS关键词 | ROLLING CIRCLE AMPLIFICATION ; EXPRESSION ; MIRNA-21 ; STRATEGY ; MACHINE |
| 资助项目 | National Natural Science Foundation of China[21804028] ; Key Research and Development Program of Anhui Province[202004h07020029] ; Key Research and Development Program of Anhui Province[202104a07020008] ; Natural Science Foundation of Anhui Province[1908085QC121] ; Natural Science Foundation of Anhui Province[2108085MB65] ; Natural Science Research Project for Universities from the Education Depart-ment of Anhui Province in China[KJ2020A0522] ; Natural Science Research Project for Universities from the Education Depart-ment of Anhui Province in China[KJ2019A0513] ; Natural Science Research Project for Universities from the Education Depart-ment of Anhui Province in China[KJ2019ZD37] ; Natural Science Project of Fuyang Normal University[2020FSKJ06ZD] ; Scientific Research and Innovation Team of Fuyang Normal University[KYTD202001] |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE SA |
| WOS记录号 | WOS:000793756100002 |
| 资助机构 | National Natural Science Foundation of China ; Key Research and Development Program of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Research Project for Universities from the Education Depart-ment of Anhui Province in China ; Natural Science Project of Fuyang Normal University ; Scientific Research and Innovation Team of Fuyang Normal University |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/130787]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Wu, Lijun; Xu, Jianguo |
| 作者单位 | 1.Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230009, Anhui, Peoples R China 2.Anhui Univ, Inst Phys Sci, Informat Mat & Intelligent Sensing Lab Anhui Prov, Hefei 230601, Anhui, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 4.Anhui Univ, Inst Informat Technol, Informat Mat & Intelligent Sensing Lab Anhui Prov, Hefei 230601, Anhui, Peoples R China 5.Fuyang Normal Univ, Sch Biol & Food Engn, Key Lab Embryo Dev & Reprod Regulat, Key Lab Environm Hormone & Reprod, Fuyang 236037, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 |
Wang, Qi,Li, Wenyong,Xia, Juan,et al. Stepwise tuning of a molecular beacon coupled Y probe regulates ternary DNA nanomachine-based microRNA determination [J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,363. |
| APA |
Wang, Qi,Li, Wenyong,Xia, Juan,Wu, Hai,Wu, Lijun,&Xu, Jianguo.(2022). Stepwise tuning of a molecular beacon coupled Y probe regulates ternary DNA nanomachine-based microRNA determination .SENSORS AND ACTUATORS B-CHEMICAL,363. |
| MLA |
Wang, Qi,et al." Stepwise tuning of a molecular beacon coupled Y probe regulates ternary DNA nanomachine-based microRNA determination ".SENSORS AND ACTUATORS B-CHEMICAL 363(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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