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Association of the tissue infiltrated and peripheral blood immune cell subsets with response to radiotherapy for rectal cancer

文献类型：期刊论文

作者Zhu, Min1,2; Li, Xingjie3; Cheng, Xu1; Yi, Xingxu1; Ye, Fang1; Li, Xiaolai4; Hu, Zongtao1; Zhang, Liwei1; Nie, Jinfu1,2; Li, Xueling1,2
刊名BMC MEDICAL GENOMICS
出版日期2022-05-09
卷号15
关键词Cancer immune microenvironment gene expression profile deconvolution Cancer infiltrated immune cell subset Peripheral blood immune cell subset Response to radiotherapy
DOI10.1186/s12920-022-01252-6
通讯作者Zhang, Liwei(doctorzhangliwei@163.com) ; Nie, Jinfu(jeffnie@cmpt.ac.cn) ; Li, Xueling(xlli@cmpt.ac.cn)
英文摘要Background: Tumor microenvironment plays pivotal roles in carcinogenesis, cancer development and metastasis. Composition of cancer immune cell subsets can be inferred by deconvolution of gene expression profile accurately. Compositions of the cell types in cancer microenvironment including cancer infiltrating immune and stromal cells have been reported to be associated with the cancer outcomes markers for cancer prognosis. However, rare studies have been reported on their association with the response to preoperative radiotherapy for rectal cancer. Methods: In this paper, we deconvoluted the immune/stromal cell composition from the gene expression profiles. We compared the composition of immune/stromal cell types in the RT responsive versus nonresponsive for rectal cancer. We also compared the peripheral blood immune cell subset composition in the stable diseases versus progressive diseases of rectal cancer patients with fluorescence-activated cell sorting from our institution. Results: Compared with the non-responsive group, the responsive group showed higher proportions of CD4(+) T cell (0.1378 +/- 0.0368 vs. 0.1071 +/- 0.0373, p = 0.0215), adipocytes, T cells CD4 memory resting, and lower proportions of CD8(+) T cell (0.1798 +/- 0.0217 vs. 0.2104 +/- 0.0415, p = 0.0239), macrophages M2, and preadipocytes in their cancer tissue. The responsive patients showed a higher ratio of CD4(+)/CD8(+) T cell proportions (mean 0.7869 vs. 0.5564, p = 0.0210). Consistently, the peripheral blood dataset showed higher proportion of CD4(+) T cells and higher ratio of CD4(+)/CD8(+) T cells, and lower proportion of CD8(+) T cells for favorable prognosis. We validated these results with a pooled dataset of GSE3493 and GSE35452, and more peripheral blood data, respectively. Finally, we imported these eight cell features including eosinophils and macrophage M1 to Support Vector Machines and could predict the pre-radiotherapy responsive versus non-responsive with an accuracy of 76%, ROC AUC 0.77, 95% confidential interval of 0.632-0.857, better than the gene signatures. Conclusions: Our results showed that the proportions of tumor-infiltrating subsets and peripheral blood immune cell subsets can be important immune cell markers and treatment targets for outcomes of radiotherapy for rectal cancer.
WOS关键词COLORECTAL-CANCER ; TUMOR RESPONSE ; EXPRESSION ; RADIATION ; LYMPHOCYTES ; SENSITIVITY ; MECHANISMS ; PREDICTION ; RESISTANCE ; PROGNOSIS
资助项目National Natural Science Foundation of China[31371340] ; National Natural Science Foundation of China[61673369] ; National Natural Science Foundation of China[61273324] ; Grant of the President Foundation of Hefei Institutes of Physical Science[YZJJ2022QN43]
WOS研究方向Genetics & Heredity
语种英语
出版者BMC
WOS记录号WOS:000792616100001
资助机构National Natural Science Foundation of China ; Grant of the President Foundation of Hefei Institutes of Physical Science
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/130825]  
专题中国科学院合肥物质科学研究院
通讯作者Zhang, Liwei; Nie, Jinfu; Li, Xueling
作者单位1.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Hlth & Med Technol, Anhui Prov Key Lab Med Phys & Technol, 350 Shushanhu Rd, Hefei 230031, Peoples R China
3.Anhui Univ, Inst Phys Sci & Informat Technol, 111 Jiulong Rd, Hefei 230601, Peoples R China
4.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei Inst Intelligent Machines, 350 Shushanhu Rd, Hefei 230031, Peoples R China
推荐引用方式
GB/T 7714		 Zhu, Min,Li, Xingjie,Cheng, Xu,et al. Association of the tissue infiltrated and peripheral blood immune cell subsets with response to radiotherapy for rectal cancer[J]. BMC MEDICAL GENOMICS,2022,15.
APA Zhu, Min.,Li, Xingjie.,Cheng, Xu.,Yi, Xingxu.,Ye, Fang.,...&Li, Xueling.(2022).Association of the tissue infiltrated and peripheral blood immune cell subsets with response to radiotherapy for rectal cancer.BMC MEDICAL GENOMICS,15.
MLA Zhu, Min,et al."Association of the tissue infiltrated and peripheral blood immune cell subsets with response to radiotherapy for rectal cancer".BMC MEDICAL GENOMICS 15(2022).

入库方式： OAI收割

来源：合肥物质科学研究院

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