A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells
文献类型：期刊论文
| 作者 | Yu, Fabiao1,2; Song, Ping1; Li, Peng1; Wang, Bingshuai1; Han, Keli1 |
| 刊名 | analyst
 |
| 出版日期 | 2012 |
| 卷号 | 137期号:16页码:3740-3749 |
| 产权排序 | 1,1 |
| 通讯作者 | 韩克利 |
| 英文摘要 | we present the design, synthesis, spectroscopy, and biological applications of pybor, a new type of fluorescent probe for peroxynitrite detection in aqueous solution and living cells. the probe employs pyrene as the fluorophore, and is equipped with a chemically responsive unit boronate. the fluorescent probe can selectively detect peroxynitrite with fluorimetric determination and high-performance liquid chromatography analyses in aqueous solution and raw264.7 cells intracellular free extracts. we also study our probe to time dependent peroxynitrite release from 3-morpholinylsydnonimine hydrochloride. confocal microscopy experiments using mouse macrophage cell line raw264.7 show that pybor is able to detect the different intracellular peroxynitrite levels. in addition, we have performed quantum chemical calculations with td-dft/m06/tzvp level with cosmo solvation model basis sets using a suite of gaussian 09 programs to provide insights into the structure optical properties of pybor and pyoh. |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | nitric-oxide ; reactive oxygen ; luminol chemiluminescence ; biological-systems ; hydrogen-peroxide ; model systems ; off-on ; acid ; superoxide ; kinetics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000306483000021 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117989]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China 2.Dalian Univ Technol, Sch Chem Engn, Dalian 116024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Fabiao,Song, Ping,Li, Peng,et al. A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells[J]. analyst,2012,137(16):3740-3749. |
| APA | Yu, Fabiao,Song, Ping,Li, Peng,Wang, Bingshuai,&Han, Keli.(2012).A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells.analyst,137(16),3740-3749. |
| MLA | Yu, Fabiao,et al."A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells".analyst 137.16(2012):3740-3749. |
入库方式： OAI收割
来源：大连化学物理研究所
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