3,3 '-Diindolylmethane attenuates inflammation and fibrosis in radiation-induced lung injury by regulating NF-kappa B/TGF-beta/Smad signaling pathways
文献类型：期刊论文
| 作者 | Zhou, Xia1,2; Bao, Wu-An2; Zhu, Xiang1; Lin, Juan1; Fan, Ju-Fen2; Yang, Yang1; Du, Xiang-Hui1,2; Wang, Yue-Zhen1,2 |
| 刊名 | EXPERIMENTAL LUNG RESEARCH
 |
| 出版日期 | 2022-03-16 |
| 卷号 | 48 |
| ISSN号 | 0190-2148 |
| 关键词 | 3,3 '-Diindolylmethane inflammatory NF-kappa B pulmonary fibrosis radiation-induced lung injury TGF beta/Smad pathway |
| DOI | 10.1080/01902148.2022.2052208 |
| 通讯作者 | Du, Xiang-Hui(duxh@zjcc.org.cn) ; Wang, Yue-Zhen(Wangyz@zjcc.org.cn) |
| 英文摘要 | Objective: This study aims to investigate the protective effect of 3,3'-diindolylmethane (DIM) on the radiation-induced lung injury (RILI) model and to explore its possible mechanism. Methods: A mouse model of RILI was established by thoracic irradiation, and dexamethasone was used as a positive drug to investigate the effect of DIM on RILI mice. Lung histopathology was analyzed by HE staining and Masson staining. Then the levels of inflammatory cytokines (TGF-beta, TNF-alpha, IL-1 beta, and IL-6), inflammatory cell counts, and activity of MPO were detected. The expression of TGF beta 1/Smad signaling pathway-related proteins was determined by immunohistochemistry. qPCR was used to analyze the mRNA expression levels of inflammatory factors, alpha-SMA and COL1A1. The expression of COX-2, NF-kappa B, I kappa Ba, PI3K, and Akt proteins was assessed by Western blot. Results: Histopathological staining of lung tissues showed that DIM administration alleviated the pulmonary inflammation and fibrosis caused by RILL Moreover, the content of inflammatory factors such as IL-1 beta and IL-6, the expression of NF-kappa B pathway-related proteins, and the counts of inflammatory cells were inhibited in lung tissue, indicating that DIM can inhibit the NF-kappa B pathway to reduce inflammation. In addition, DIM could down-regulate the mRNA levels of alpha-SMA, COL1A1, and downregulate TGF beta 1, Smad3, and p-Smad2/3 in lung tissues. Conclusion: Our study confirms that DIM has the potential to treat RILI in vivo by inhibiting fibrotic and inflammatory responses in lung tissue through the TGF beta/Smad and NE-kappa B dual pathways, respectively. [GRAPHICS] . |
| WOS关键词 | CELLS ; INVOLVEMENT |
| 资助项目 | Zhejiang Provincial Medicinal Health Program[2020KY469] ; Zhejiang Provincial Medicinal Health Program[2021KY097] ; Science and Technology Program of Traditional Chinese medicine in Zhejiang Province[2020ZA021] ; Science and Technology Program of Traditional Chinese medicine in Zhejiang Province[2021ZA022] |
| WOS研究方向 | Respiratory System |
| 语种 | 英语 |
| 出版者 | TAYLOR & FRANCIS INC |
| WOS记录号 | WOS:000799555600001 |
| 资助机构 | Zhejiang Provincial Medicinal Health Program ; Science and Technology Program of Traditional Chinese medicine in Zhejiang Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/130935]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Du, Xiang-Hui; Wang, Yue-Zhen |
| 作者单位 | 1.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou, Zhejiang, Peoples R China 2.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Radiat Therapy, Hangzhou, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Xia,Bao, Wu-An,Zhu, Xiang,et al. 3,3 '-Diindolylmethane attenuates inflammation and fibrosis in radiation-induced lung injury by regulating NF-kappa B/TGF-beta/Smad signaling pathways[J]. EXPERIMENTAL LUNG RESEARCH,2022,48. |
| APA | Zhou, Xia.,Bao, Wu-An.,Zhu, Xiang.,Lin, Juan.,Fan, Ju-Fen.,...&Wang, Yue-Zhen.(2022).3,3 '-Diindolylmethane attenuates inflammation and fibrosis in radiation-induced lung injury by regulating NF-kappa B/TGF-beta/Smad signaling pathways.EXPERIMENTAL LUNG RESEARCH,48. |
| MLA | Zhou, Xia,et al."3,3 '-Diindolylmethane attenuates inflammation and fibrosis in radiation-induced lung injury by regulating NF-kappa B/TGF-beta/Smad signaling pathways".EXPERIMENTAL LUNG RESEARCH 48(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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