Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion
文献类型：期刊论文
| 作者 | Jiang, Bo1,2,3; Yang, Kaiguang1; Zhao, Qun1,2; Wu, Qi1,2; Liang, Zhen1; Zhang, Lihua1; Peng, Xiaojun3; Zhang, Yukui1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2012-09-07 |
| 卷号 | 1254页码:8-13 |
| 关键词 | Immobilized enzymatic reactor Graphene oxide Magnetic nanoparticles Proteomics |
| 产权排序 | 1,1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | in this paper, magnetic fe3o4 nanoparticles modified graphene oxide nanocomposites (go-co-nh-fe3o4) were prepared by covalent bonding, via the reaction between the amino groups of fuctionalized fe3o4 and the carboxylic groups of go, confirmed by fourier-transform infrared spectra, raman spectroscopy, and transmission electron microscopy. with go-co-nh-fe3o4 as a novel substrate, trypsin was immobilized via pi-pi stacking and hydrogen bonding interaction, and the binding capacity of trypsin reached as high as 0.275 mg/mg. since go-co-nh-fe3o4 worked as not only support for enzyme immobilization, but also as an excellent microwave irradiation absorber, the digestion efficiency could be further improved with microwave assistance. by such an immobilized enzymatic reactor (imer), standard proteins could be efficiently digested within 15 s, with sequence coverages comparable or better than those obtained by conventional in-solution digestion (12 h). since trypsin was immobilized under mild conditions, the enzymatic activity of imer preserved at least for a month. in addition, due to the good hydrophilicity of go, no peptide residue was observed in the sequent digestion of bovine serum albumin and myoglobin. to further confirm the efficiency of such an imer for proteome analysis, it was applied to digest proteins extracted from rat liver, followed by nanorplc-esi-ms/ms analysis. with only 5 min microwave-assisted digestion, in 3 parallel runs, totally 456 protein groups were identified, comparable to that obtained by 12 h in-solution digestion, indicating the great potential of imers with go-co-nh-fe3o4 as the support for high throughput proteome study. (c) 2012 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | on-plate proteolysis ; mass-spectrometry ; ms analysis ; silica ; nanoparticles ; online ; microreactor ; fabrication ; monoliths ; sheets |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000308048000002 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/117993]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Bo,Yang, Kaiguang,Zhao, Qun,et al. Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion[J]. journal of chromatography a,2012,1254:8-13. |
| APA | Jiang, Bo.,Yang, Kaiguang.,Zhao, Qun.,Wu, Qi.,Liang, Zhen.,...&Zhang, Yukui.(2012).Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion.journal of chromatography a,1254,8-13. |
| MLA | Jiang, Bo,et al."Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion".journal of chromatography a 1254(2012):8-13. |
入库方式： OAI收割
来源：大连化学物理研究所
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