Construction of a ceRNA Network and Comprehensive Analysis of lncRNA in Hepatocellular Carcinoma
文献类型：期刊论文
| 作者 | Wang, Lin1,2; Zhao, Jun2; Zhu, Cancan2 ; Yang, Ke2; Zhu, Ling2; Liu, Yong2
|
| 刊名 | GENES
 |
| 出版日期 | 2022-05-01 |
| 卷号 | 13 |
| 关键词 | hepatocellular carcinoma competing endogenous RNA mRNA-miRNA-lncRNA bioinformatics analysis |
| DOI | 10.3390/genes13050785 |
| 通讯作者 | Liu, Yong(liuyong@aiofm.ac.cn) |
| 英文摘要 | To explore the RNA biomolecular marker associated with hepatocellular carcinoma (HCC) prognosis, we constructed a regulatory network of competitive endogenous RNAs (ceRNAs), which provides favorable conditions for the early diagnosis, prognostic monitoring, and personalized treatment of HCC. In this study, the differentially expressed genes (DEGs) of patients with HCC were obtained from the Gene Expression Omnibus. We identified 574 upregulated genes and 274 downregulated genes relevant to HCC occurrence (p < 0.05). Subsequently, we constructed the protein-protein interaction (PPI) network using these DEGs and identified the hub genes from the PPI. We then determined the expression and prognostic values of the hub genes from the GEPIA and Kaplan-Meier plotter databases. After the upstream microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) were respectively identified by miRTarBase and miRNet, we validated the expression of the key miRNAs in the serum using qPCR experiments. Moreover, we identified a two-lncRNA (LINC01184 and ADORA2A-AS1) signature from the upstream lncRNA that effectively predicted overall survival and had promotive effects for HCC. To verify the clinical significance of the signature, we validated the expression of the lncRNA in HCC tissues. Finally, we discovered and identified four mRNAs, four miRNAs, and five lncRNAs associated with the prognosis of HCC and constructed a new ceRNA regulatory network, which will be beneficial for the accurate diagnosis and treatment of HCC. |
| WOS关键词 | CANCER ; CELLS |
| 资助项目 | Natural Science Foundation of Anhui Province of China[2108085QC96] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2018489] |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000802466600001 |
| 资助机构 | Natural Science Foundation of Anhui Province of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/131151]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Liu, Yong |
| 作者单位 | 1.Univ Sci & Technol China, Grad Sch, Sci Isl Branch, Hefei 230026, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Inst Opt & Fine Mech, Hefei 230031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Lin,Zhao, Jun,Zhu, Cancan,et al. Construction of a ceRNA Network and Comprehensive Analysis of lncRNA in Hepatocellular Carcinoma[J]. GENES,2022,13. |
| APA | Wang, Lin,Zhao, Jun,Zhu, Cancan,Yang, Ke,Zhu, Ling,&Liu, Yong.(2022).Construction of a ceRNA Network and Comprehensive Analysis of lncRNA in Hepatocellular Carcinoma.GENES,13. |
| MLA | Wang, Lin,et al."Construction of a ceRNA Network and Comprehensive Analysis of lncRNA in Hepatocellular Carcinoma".GENES 13(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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