Site-Specific F-19 NMR Method for Detecting Arf6 GEF Activity
文献类型：期刊论文
| 作者 | Wang, Jiarong2 ; Wu, Bo2; Zhang, Youjia1,2; Ge, Liang2; Wang, Junfeng2,3
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| 刊名 | ANALYTICAL CHEMISTRY
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| 出版日期 | 2022-06-14 |
| 卷号 | 94 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.1c05563 |
| 通讯作者 | Wu, Bo(wubo@hmfl.ac.cn) ; Wang, Junfeng(junfeng@hmfl.ac.cn) |
| 英文摘要 | Guanine nucleotide exchange factors (GEFs) of small GTPase (sGTPase) coordinate signal networks in normal cells and dysfunction in cancer. Therefore, effective monitoring of GEF activity is very important for studying the regulation of sGTPase signal transduction. In this study, we developed a 1D F-19 NMR-based method for rapid detection of the GEF activity of sGTPases. The activity of Arf6GEF in vitro and cell lysate environment can be conveniently detected by tracking the conformational changes of the Arf6 switch region where a tfmF site-specific F-19 labeling at Phe47 was introduced. This strategy could potentially be applied to monitor the conformational change of Arf6 or other sGTPase and detect the activities of sGTPase regulatory proteins in physiology and pathology environments. |
| WOS关键词 | GUANINE-NUCLEOTIDE EXCHANGE ; ACTIVATION ; PROTEINS ; PROBE |
| 资助项目 | National Natural Science Foundation of China[21673244] ; Collaborative Innovation Program of Hefei Science Center, CAS[2020HSC-CIP008] ; High Magnetic Field Laboratory of Anhui Province |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000811922800001 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Natural Science Foundation of China ; Collaborative Innovation Program of Hefei Science Center, CAS ; High Magnetic Field Laboratory of Anhui Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/131267]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Wu, Bo; Wang, Junfeng |
| 作者单位 | 1.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China 3.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jiarong,Wu, Bo,Zhang, Youjia,et al. Site-Specific F-19 NMR Method for Detecting Arf6 GEF Activity[J]. ANALYTICAL CHEMISTRY,2022,94. |
| APA | Wang, Jiarong,Wu, Bo,Zhang, Youjia,Ge, Liang,&Wang, Junfeng.(2022).Site-Specific F-19 NMR Method for Detecting Arf6 GEF Activity.ANALYTICAL CHEMISTRY,94. |
| MLA | Wang, Jiarong,et al."Site-Specific F-19 NMR Method for Detecting Arf6 GEF Activity".ANALYTICAL CHEMISTRY 94(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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