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Label-Free Detection and Translocation Dynamics Study of Single-Molecule Herceptin Using Solid-State Nanopores

文献类型：期刊论文

作者Hu, Rui2,3; Lu, Wenlong2,3; Wei, Guanghao2,3; Nan, Hexin4; Li, Juan4; Zhao, Qing1,2,3
刊名ADVANCED MATERIALS TECHNOLOGIES
出版日期2022-05-31
ISSN号2365-709X
关键词solid-state nanopore single-molecule detection protein dynamics monoclonal antibody protein unfolding
DOI10.1002/admt.202200018
通讯作者Hu, Rui(hurui1991@live.cn) ; Zhao, Qing(zhaoqing@pku.edu.cn)
英文摘要Herceptin (or trastuzumab) is an important therapeutic monoclonal antibody (mAb) used in the treatment of HER2-positive breast cancer. Real-time counting and characterization of Herceptin is a fundamental step in the field of disease-related diagnosis and therapy. Solid-state nanopore-based biosensors have been proved to hold great potential in characterizing the properties of proteins at the single-molecule level for in vitro diagnosis. Here, the label-free detection and detailed translocation dynamics study of Herceptin using solid-state nanopores are demonstrated. By constricting nanopore size close to the size of Herceptin, the detection sensitivity and temporal resolution have been significantly improved, allowing the delicate probing of the structural information of single-molecule Herceptin. Therefore, three types of Herceptin translocation events are identified through nanopores, single-level, multi-level and spike-like events, emerged at different voltages regimes, indicating the unfolding kinetics of Herceptin under electric field. The potential influence of a high electric field on complex biomolecules is highlighted and a novel prospective platform is provided for label-free detection of single-molecule therapeutic monoclonal antibodies via solid-state nanopores as a miniaturized biomedical device.
WOS关键词METASTATIC BREAST-CANCER ; MONOCLONAL-ANTIBODY ; PROTEINS ; TRASTUZUMAB ; IMMUNOGLOBULIN ; CHEMOTHERAPY ; FLEXIBILITY ; VINORELBINE ; BANDWIDTH ; ELISA
资助项目National Key Research and Development Program of China[2019YFA0707003] ; National Key Research and Development Program of China[2019YFE0114100] ; National Key Research and Development Program of China[2020YFA0909000] ; National Natural Science Foundation of China[NSFC 51872007] ; National Natural Science Foundation of China[NSFC 52103296] ; National Natural Science Foundation of China[NSFC 91959102] ; Joint Laboratory of Offshore Wind Power and Intelligent Energy System[HNKJ20-H88] ; Beijing Municipal Natural Science Foundation[7202094] ; Shanghai Rising-Star Program[19QA1405400]
WOS研究方向Materials Science
语种英语
出版者WILEY
WOS记录号WOS:000803014100001
资助机构National Key Research and Development Program of China ; National Natural Science Foundation of China ; Joint Laboratory of Offshore Wind Power and Intelligent Energy System ; Beijing Municipal Natural Science Foundation ; Shanghai Rising-Star Program
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/131333]  
专题中国科学院合肥物质科学研究院
通讯作者Hu, Rui; Zhao, Qing
作者单位1.Peking Univ, Yangtze Delta Inst Optoelect, Nantong 226010, Jiangsu, Peoples R China
2.Peking Univ, Sch Phys, State Key Lab Mesoscop Phys, Beijing 100871, Peoples R China
3.Peking Univ, Sch Phys, Frontiers Sci Ctr Nanooptoelect, Beijing 100871, Peoples R China
4.Chinese Acad Sci, Univ Chinese Acad Sci, Canc Hosp, Zhejiang Canc Hosp,Inst Basic Med & Canc IBMC, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Hu, Rui,Lu, Wenlong,Wei, Guanghao,et al. Label-Free Detection and Translocation Dynamics Study of Single-Molecule Herceptin Using Solid-State Nanopores[J]. ADVANCED MATERIALS TECHNOLOGIES,2022.
APA Hu, Rui,Lu, Wenlong,Wei, Guanghao,Nan, Hexin,Li, Juan,&Zhao, Qing.(2022).Label-Free Detection and Translocation Dynamics Study of Single-Molecule Herceptin Using Solid-State Nanopores.ADVANCED MATERIALS TECHNOLOGIES.
MLA Hu, Rui,et al."Label-Free Detection and Translocation Dynamics Study of Single-Molecule Herceptin Using Solid-State Nanopores".ADVANCED MATERIALS TECHNOLOGIES (2022).

入库方式： OAI收割

来源：合肥物质科学研究院

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