Substrate topography regulates extracellular matrix component secretion by bone marrow-derived mesenchymal stem cells
文献类型：期刊论文
| 作者 | Liu, Xiaozhen1,2; Ren, Zhuozhuo3,4; Meng, Xuli5; Xu, Yong6 |
| 刊名 | JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
 |
| 出版日期 | 2022-06-01 |
| 卷号 | 7 |
| 关键词 | Mesenchymal stem cells Electro-spinning Cell culture substrate Poly-L-lactic acid nano-fibers Extracellular matrix |
| ISSN号 | 2468-2284 |
| DOI | 10.1016/j.jsamd.2022.100437 |
| 通讯作者 | Meng, Xuli(mxlmail@126.com) ; Xu, Yong(yong.xu@tu-dresden.de) |
| 英文摘要 | The extracellular matrix (ECM) has a complicated nano- and macro-architecture. We prepared poly-L-lactic acid (PLLA)-ECM substrates derived from mesenchymal stem cells (MSCs) by culturing cells on PLLA electro-spun fiber membranes of three different diameters. After this, PLLA membranes made with electro-spun nano-fibers were more suitable than traditional tissue culture films for cell proliferation capacity and morphology. In addition, membranes comprising these fibers promoted the gene expression of biomolecules related to ECM composition and production when compared with gene expression using the conventional substrate culture method. MSC proliferation on nano-fibrous PLLA-ECM substrates showed excellent biocompatibility. Moreover, these MSCs also expressed higher levels of the MSC markers, CD44, CD90, and CD105, as indicated by flow cytometry. Thus, PLLA electro-spun fibers are promising substrates for MSCs and promote ECM secretion, suggesting this method may be used for large-scale stem cell expansion. (C) 2022 Vietnam National University, Hanoi. Published by Elsevier B.V. |
| WOS关键词 | DERMAL MATRIX ; ECM ; DIFFERENTIATION ; SCAFFOLDS ; CULTURE ; DESIGN |
| 资助项目 | Zhejiang Medical and Health Research Project[2020KY461] ; National Natural Science Foun-dation of China[81973861] ; National Health Commission Sci-entific Research Fund-Zhejiang Provincial Health and Health Major Science and Technology Plan Project |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000787899200007 |
| 出版者 | VIETNAM NATL UNIV |
| 资助机构 | Zhejiang Medical and Health Research Project ; National Natural Science Foun-dation of China ; National Health Commission Sci-entific Research Fund-Zhejiang Provincial Health and Health Major Science and Technology Plan Project |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/131446]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Meng, Xuli; Xu, Yong |
| 作者单位 | 1.Hangzhou Med Coll, Affiliated Peoples Hosp, Zhejiang Prov Peoples Hosp, Dept Breast Surg,Dept Head & Neck Surg, Hangzhou 310014, Zhejiang, Peoples R China 2.Key Lab Diag & Treatment Endocrine Gland Dis Zhej, Hangzhou 310014, Peoples R China 3.Chinese Acad Sci, Inst Canc & Basic Med ICBM, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Med Engn, Beijing, Peoples R China 5.Hangzhou Med Coll, Affiliated Peoples Hosp, Zhejiang Prov Peoples Hosp, Dept Breast Surg, Hangzhou 310014, Zhejiang, Peoples R China 6.Tech Univ Dresden, B CUBE Ctr Mol Bioengn, D-01307 Dresden, Germany |
| 推荐引用方式 GB/T 7714 | Liu, Xiaozhen,Ren, Zhuozhuo,Meng, Xuli,et al. Substrate topography regulates extracellular matrix component secretion by bone marrow-derived mesenchymal stem cells[J]. JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES,2022,7. |
| APA | Liu, Xiaozhen,Ren, Zhuozhuo,Meng, Xuli,&Xu, Yong.(2022).Substrate topography regulates extracellular matrix component secretion by bone marrow-derived mesenchymal stem cells.JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES,7. |
| MLA | Liu, Xiaozhen,et al."Substrate topography regulates extracellular matrix component secretion by bone marrow-derived mesenchymal stem cells".JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES 7(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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