Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes
文献类型：期刊论文
| 作者 | Wang, Fangjun; Zhu, Jun; Hu, Lianghai; Qin, Hongqiang; Ye, Mingliang; Zou, Hanfa |
| 刊名 | protein & cell
 |
| 出版日期 | 2012-09-01 |
| 卷号 | 3期号:9页码:669-674 |
| 关键词 | human serum endogenous peptide N and C termini disease diagnosis |
| ISSN号 | 1674-800x |
| 产权排序 | 1,1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | the human serum proteome is closely associated with the state of the body. endogenous peptides derived from proteolytic enzymes cleaving on serum proteins are widely studied due to their potential application in disease-specific marker discovery. however, the reproducibility of peptidome analysis of endogenous peptides is significantly influenced by the proteolytic enzymes within body fluids, thereby limiting the clinical use of the endogenous peptides. we comprehensively investigated the n and c terminus of endogenous peptides using peptidomics. the cleavage site patterns of the n and c terminus and adjacent sites from all the identified endogenous peptides were highly conserved under different sample preparation conditions, including long-term incubation at 37 degrees c and pretreatment with repeated freeze-thaw cycles. furthermore, a distinguishable cleavage site pattern was obtained when a different disease serum was analyzed. the conserved cleavage site pattern derived from proteolytic enzymes holds potential in highly specific disease diagnosis. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | cell biology |
| 研究领域[WOS] | cell biology |
| 关键词[WOS] | mass-spectrometry ; proteome ; plasma ; quantification ; peptidomics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310532800004 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/118013]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fangjun,Zhu, Jun,Hu, Lianghai,et al. Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes[J]. protein & cell,2012,3(9):669-674. |
| APA | Wang, Fangjun,Zhu, Jun,Hu, Lianghai,Qin, Hongqiang,Ye, Mingliang,&Zou, Hanfa.(2012).Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes.protein & cell,3(9),669-674. |
| MLA | Wang, Fangjun,et al."Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes".protein & cell 3.9(2012):669-674. |
入库方式： OAI收割
来源：大连化学物理研究所
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