Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice
文献类型：期刊论文
| 作者 | Yang, Miaomiao2,3,4; Fan, Qiang2,3; Hei, Tom K.6; Chen, Guodong2; Cao, Wei2,3; Meng, Gang4 ; Han, Wei1,2,5
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| 刊名 | ANTIOXIDANTS
 |
| 出版日期 | 2022-08-01 |
| 卷号 | 11 |
| 关键词 | radiation pneumonitis (RP) single-cell RNA-seq (scRNA-seq) mouse oxidative stress |
| DOI | 10.3390/antiox11081457 |
| 通讯作者 | Han, Wei(hanw@hfcas.ac.cn) |
| 英文摘要 | Radiation-induced lung injury (RILI), especially radiation pneumonitis (RP), is a common clinical complication associated with thoracic radiotherapy for malignant tumors. However, the specific contributions of each cell subtype to this process are unknown. Here, we provide the single-cell pathology landscape of the RP in a mouse model by unbiased single-cell RNA-seq (scRNA-seq). We found a decline of type 2 alveolar cells in the RP lung tissue, with an expansion of macrophages, especially the Fabp4low and Spp1high subgroup, while Fabp4high macrophages were almost depleted. We observed an elevated expression of multiple mitochondrial genes in the RP group, indicating a type 2 alveolar cell (AT2) response to oxidative stress. We also calculated the enrichment of a cGAS-STING signaling pathway, which may be involved in regulating inflammatory responses and cancer progression in AT2 cells of PR mice. We delineate markers and transcriptional states, identify a type 2 alveolar cell, and uncover fundamental determinants of lung fibrosis and inflammatory response in RP lung tissue of mice. |
| WOS关键词 | LUNG ENDOTHELIAL-CELLS ; PULMONARY-FIBROSIS ; INJURY ; EXPRESSION ; CANCER |
| 资助项目 | National Natural Science Foundation of China[81974484] ; National Natural Science Foundation of China[U20A20372] ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[2019HSC-CIP010] ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection |
| WOS研究方向 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Food Science & Technology |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000846315000001 |
| 资助机构 | National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/131814]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Han, Wei |
| 作者单位 | 1.Soochow Univ, Collaborat Innovat Ctr Radiat Med Jiangsu Higher, Suzhou 215123, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Inst Hlth & Med Technol, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China 4.Anhui Med Univ, Clin Pathol Ctr, Affiliated Hosp 4, Hefei 230022, Peoples R China 5.Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou 215123, Peoples R China 6.Columbia Univ, Coll Phys & Surg, Ctr Radiol Res, Med Ctr, New York, NY 10032 USA |
| 推荐引用方式 GB/T 7714 | Yang, Miaomiao,Fan, Qiang,Hei, Tom K.,et al. Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice[J]. ANTIOXIDANTS,2022,11. |
| APA | Yang, Miaomiao.,Fan, Qiang.,Hei, Tom K..,Chen, Guodong.,Cao, Wei.,...&Han, Wei.(2022).Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice.ANTIOXIDANTS,11. |
| MLA | Yang, Miaomiao,et al."Single-Cell Transcriptome Analysis of Radiation Pneumonitis Mice".ANTIOXIDANTS 11(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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