Simulating androgen receptor selection in designer yeast
文献类型：期刊论文
| 作者 | Zhang, Haoran5,6,7; Zhang, Lu5,6,7 ; Xu, Yipeng4; Chen, Shaoyong2,3; Ma, Zhenyi1,7; Yao, Mingdong5,6,7; Li, Fangyin4; Li, Bo5,6,7; Yuan, Yingjin5,6,7
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| 刊名 | SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
 |
| 出版日期 | 2022-12-01 |
| 卷号 | 7 |
| 关键词 | Androgen receptor mutations Androgen receptor antagonists Prostate cancer Saccharomyces cerevisiae |
| ISSN号 | 2405-805X |
| DOI | 10.1016/j.synbio.2022.07.005 |
| 通讯作者 | Yuan, Yingjin(yjyuan@tju.edu.cn) |
| 英文摘要 | Androgen receptor (AR) mutation is closely associated with prostate cancer (PCa) and is one of the mechanisms of resistance to PCa therapies such as AR antagonists. Although sequencing technologies like next-generation sequencing (NGS) contributes to the high-throughput and precise detection of AR mutations carried by PCa patients, the lack of interpretations of these clinical genetic variants has still been a roadblock for PCa-targeted precision medicine. Here, we established a designer yeast reporter assay to simulate natural androgen receptor (AR) selection using AR antagonists. Yeast HIS3 gene transactivation was associated with the ligand-induced recruitment of steroid receptor coactivator-1 (SRC-1) by AR mutants, where yeast growth in histidine-free me-dium was determined as the outcome. This assay is applicable to determine a wide range of clinical AR mutants including those with loss of function relating to androgen insensitivity syndrome (AIS), and those associated with PCa conferring resistance to AR antagonists such as enzalutamide (ENZ), bicalutamide (BIC), and cyproterone acetate (CPA). One clinical AR mutant previously reported to confer ENZ-resistance, F877L, was found to confer partial resistance to CPA as well using designer yeast. Our simple and efficient assay can enable precise one-pot screening of AR mutants, providing a reference for tailored medicine. |
| WOS关键词 | RESISTANT PROSTATE-CANCER ; STRUCTURAL BASIS ; BINDING DOMAIN ; CELL-GROWTH ; GENE ; MECHANISMS ; ENZALUTAMIDE ; ANTIANDROGEN ; BICALUTAMIDE ; MUTATIONS |
| 资助项目 | National Natural Science Foundation of China[21621004] ; National Natural Science Foundation of China[31861143017] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000848339100001 |
| 出版者 | KEAI PUBLISHING LTD |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/131926]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Yuan, Yingjin |
| 作者单位 | 1.Tianjin Med Univ, Sch Basic Med Sci, Tianjin 300070, Peoples R China 2.Harvard Med Sch, Boston, MA 02215 USA 3.Beth Israel Deaconess Med Ctr, Dept Med, Hematol Oncol Div, Boston, MA 02215 USA 4.Univ Chinese Acad Sci, Canc Hosp, Zhejiang Canc Hosp, Dept Urol, Hangzhou, Peoples R China 5.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China 6.Tianjin Univ, Key Lab Syst Bioengn, Minist Educ, Tianjin 300072, Peoples R China 7.Tianjin Univ, Frontier Sci Ctr Synthet Biol, Tianjin 300072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Haoran,Zhang, Lu,Xu, Yipeng,et al. Simulating androgen receptor selection in designer yeast[J]. SYNTHETIC AND SYSTEMS BIOTECHNOLOGY,2022,7. |
| APA | Zhang, Haoran.,Zhang, Lu.,Xu, Yipeng.,Chen, Shaoyong.,Ma, Zhenyi.,...&Yuan, Yingjin.(2022).Simulating androgen receptor selection in designer yeast.SYNTHETIC AND SYSTEMS BIOTECHNOLOGY,7. |
| MLA | Zhang, Haoran,et al."Simulating androgen receptor selection in designer yeast".SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 7(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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